|
|
Line 33: |
Line 33: |
| <div class="h1_content"> | | <div class="h1_content"> |
| <div class="pic_box"> | | <div class="pic_box"> |
− | <strong>OD600 reference point</strong><br /><div align="center"> | + | <strong>OD600 reference point</strong><br /> |
− | <table border="1"> | + | <img src="https://static.igem.org/mediawiki/2017/8/85/T--Jilin_China--interlab1.png" /> |
− | <tr>
| + | |
− | <th></th> <th>LUDOX-HS40</th> <th>H2O</th>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 1</td> <td>0.047</td> <td>0.04</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 2</td> <td>0.045</td> <td>0.04</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 3</td> <td>0.048</td> <td>0.042</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 4</td> <td>0.048</td> <td>0.041</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Arith. Mean</td> <td>0.047</td> <td>0.04075</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Corrected Abs600</td> <td>0.00625</td> <td></td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Reference OD600</td> <td>0.0425</td> <td></td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>OD600/Abs600</td> <td>6.8</td> <td></td>
| + | |
− | </tr>
| + | |
− | </table></div>
| + | |
| </div> | | </div> |
| <div class="pic_box"> | | <div class="pic_box"> |
| <strong>Fluorescein standard curve</strong><br /> | | <strong>Fluorescein standard curve</strong><br /> |
− | <div align="center"> | + | <img src="https://static.igem.org/mediawiki/2017/4/4f/T--Jilin_China--interlab2.png" /><br /><br /> |
− | <table border="1">
| + | |
− | <tr>
| + | |
− | <th>uM Fluorescein</th> <th>50</th> <th>25</th> <th>12.5</th> <th>6.25</th> <th>3.125</th> <th>1.5625</th> <th>0.78125</th> <th>0.39063</th> <th>0.19531</th> <th>0.097656</th> <th>0.048828</th> <th>0</th>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 1</td> <td>40649</td> <td>26105</td> <td>16044</td> <td>8715</td> <td>4461</td> <td>2275</td> <td>1197</td> <td>594</td> <td>298</td> <td>160</td> <td>76</td> <td>4</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 2</td> <td>41111</td> <td>26716</td> <td>15422</td> <td>8594</td> <td>4540</td> <td>2273</td> <td>1170</td> <td>593</td> <td>300</td> <td>152</td> <td>77</td> <td>4</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 3</td> <td>41157</td> <td>26528</td> <td>15611</td> <td>8410</td> <td>4472</td> <td>2280</td> <td>1159</td> <td>629</td> <td>277</td> <td>145</td> <td>76</td> <td>5</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Replicate 4</td> <td>40760</td> <td>26858</td> <td>15662</td> <td>8607</td> <td>4392</td> <td>2340</td> <td>1174</td> <td>591</td> <td>297</td> <td>157</td> <td>80</td> <td>4</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Arith. Mean</td> <td>40919.25</td> <td>26551.75</td> <td>15684.75</td> <td>8581.5</td> <td>4466.25</td> <td>2292</td> <td>1175</td> <td>601.75</td> <td>293</td> <td>153.5</td> <td>77.25</td> <td>4.25</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td>Arith. Std.Dev.</td> <td>252.7771</td> <td>327.0661</td> <td>260.8031</td> <td>126.5451</td> <td>60.5881</td> <td>32.1351</td> <td>15.9792</td> <td>18.2094</td> <td>10.7393</td> <td>6.5574</td> <td>1.893</td> <td>0.5</td>
| + | |
− | </tr>
| + | |
− | </table></div><br /><br />
| + | |
| <img src="https://static.igem.org/mediawiki/2017/f/f7/T--Jilin_China--interlab3.png" /><br /><br /> | | <img src="https://static.igem.org/mediawiki/2017/f/f7/T--Jilin_China--interlab3.png" /><br /><br /> |
| <img src="https://static.igem.org/mediawiki/2017/thumb/9/9a/T--Jilin_China--interlab_log_scale_pic.png/800px-T--Jilin_China--interlab_log_scale_pic.png" /><br /><br /> | | <img src="https://static.igem.org/mediawiki/2017/thumb/9/9a/T--Jilin_China--interlab_log_scale_pic.png/800px-T--Jilin_China--interlab_log_scale_pic.png" /><br /><br /> |
Line 96: |
Line 45: |
| <strong>Raw Plate Reader Measurements</strong><br /> | | <strong>Raw Plate Reader Measurements</strong><br /> |
| Dilution Calculation:<br /> | | Dilution Calculation:<br /> |
− | <img src="https://static.igem.org/mediawiki/2017/d/d5/T--Jilin_China--interlab_dilution1.png" /><br /> | + | <img src="https://static.igem.org/mediawiki/2017/2/26/T--Jilin_China--interlab5.jpg" /><br /><br /> |
− | <img src="https://static.igem.org/mediawiki/2017/3/30/T--Jilin_China--interlab_dilution2.png" /><br /><br />
| + | |
| Fluorescence Raw Readings:<br /><br /> | | Fluorescence Raw Readings:<br /><br /> |
− | <table border="1" style="font-size:6px;">
| |
− | <tr>
| |
− | <th>Hour 0:</th> <th>Neg. Control</th> <th>Pos. Control</th> <th>Device 1</th> <th>Device 2</th> <th>Device 3</th> <th>Device 4</th> <th>Device 5</th> <th>Device 6</th> <th>LB + Chlor (blank)</th>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 1</td> <td>0.07</td> <td>0.07</td> <td>0.062</td> <td>0.068</td> <td>0.066</td> <td>0.067</td> <td>0.069</td> <td>0.068</td> <td>0.042</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 2</td> <td>0.07</td> <td>0.068</td> <td>0.063</td> <td>0.063</td> <td>0.063</td> <td>0.065</td> <td>0.069</td> <td>0.065</td> <td>0.041</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 3</td> <td>0.069</td> <td>0.066</td> <td>0.065</td> <td>0.066</td> <td>0.065</td> <td>0.066</td> <td>0.07</td> <td>0.067</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 4</td> <td>0.072</td> <td>0.069</td> <td>0.065</td> <td>0.069</td> <td>0.067</td> <td>0.067</td> <td>0.07</td> <td>0.067</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 1</td> <td>0.065</td> <td>0.062</td> <td>0.059</td> <td>0.063</td> <td>0.064</td> <td>0.064</td> <td>0.061</td> <td>0.065</td> <td>0.043</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 2</td> <td>0.064</td> <td>0.061</td> <td>0.058</td> <td>0.063</td> <td>0.065</td> <td>0.062</td> <td>0.062</td> <td>0.063</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 3</td> <td>0.065</td> <td>0.062</td> <td>0.058</td> <td>0.062</td> <td>0.06</td> <td>0.063</td> <td>0.063</td> <td>0.062</td> <td>0.041</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 4</td> <td>0.065</td> <td>0.063</td> <td>0.059</td> <td>0.063</td> <td>0.066</td> <td>0.063</td> <td>0.063</td> <td>0.065</td> <td>0.044</td>
| |
− | </tr>
| |
− | </table><br />
| |
− | <table border="1">
| |
− | <tr>
| |
− | <th>Hour 2:</th> <th>Neg. Control</th> <th>Pos. Control</th> <th>Device 1</th> <th>Device 2</th> <th>Device 3</th> <th>Device 4</th> <th>Device 5</th> <th>Device 6</th> <th>LB + Chlor (blank)</th>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 1</td> <td>0.173</td> <td>0.162</td> <td>0.064</td> <td>0.146</td> <td>0.153</td> <td>0.132</td> <td>0.155</td> <td>0.16</td> <td>0.047</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 2</td> <td>0.188</td> <td>0.164</td> <td>0.07</td> <td>0.15</td> <td>0.158</td> <td>0.133</td> <td>0.167</td> <td>0.157</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 3</td> <td>0.18</td> <td>0.156</td> <td>0.074</td> <td>0.155</td> <td>0.166</td> <td>0.14</td> <td>0.163</td> <td>0.18</td> <td>0.051</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 4</td> <td>0.181</td> <td>0.162</td> <td>0.073</td> <td>0.155</td> <td>0.167</td> <td>0.136</td> <td>0.172</td> <td>0.184</td> <td>0.045</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 1</td> <td>0.141</td> <td>0.1</td> <td>0.063</td> <td>0.122</td> <td>0.139</td> <td>0.118</td> <td>0.132</td> <td>0.148</td> <td>0.046</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 2</td> <td>0.15</td> <td>0.101</td> <td>0.065</td> <td>0.122</td> <td>0.141</td> <td>0.121</td> <td>0.132</td> <td>0.149</td> <td>0.043</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 3</td> <td>0.148</td> <td>0.105</td> <td>0.069</td> <td>0.13</td> <td>0.136</td> <td>0.124</td> <td>0.138</td> <td>0.199</td> <td>0.043</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 4</td> <td>0.148</td> <td>0.116</td> <td>0.066</td> <td>0.137</td> <td>0.147</td> <td>0.12</td> <td>0.14</td> <td>0.153</td> <td>0.044</td>
| |
− | </tr>
| |
− | </table><br />
| |
− | <table border="1">
| |
− | <tr>
| |
− | <th>Hour 4:</th> <th>Neg. Control</th> <th>Pos. Control</th> <th>Device 1</th> <th>Device 2</th> <th>Device 3</th> <th>Device 4</th> <th>Device 5</th> <th>Device 6</th> <th>LB + Chlor (blank)</th>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 1</td> <td>0.289</td> <td>0.274</td> <td>0.093</td> <td>0.264</td> <td>0.256</td> <td>0.255</td> <td>0.266</td> <td>0.248</td> <td>0.045</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 2</td> <td>0.297</td> <td>0.282</td> <td>0.098</td> <td>0.272</td> <td>0.255</td> <td>0.264</td> <td>0.275</td> <td>0.274</td> <td>0.042</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 3</td> <td>0.307</td> <td>0.279</td> <td>0.101</td> <td>0.279</td> <td>0.271</td> <td>0.259</td> <td>0.279</td> <td>0.278</td> <td>0.046</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 4</td> <td>0.289</td> <td>0.297</td> <td>0.104</td> <td>0.281</td> <td>0.263</td> <td>0.26</td> <td>0.285</td> <td>0.283</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 1</td> <td>0.256</td> <td>0.193</td> <td>0.077</td> <td>0.222</td> <td>0.233</td> <td>0.235</td> <td>0.281</td> <td>0.245</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 2</td> <td>0.269</td> <td>0.192</td> <td>0.083</td> <td>0.239</td> <td>0.242</td> <td>0.238</td> <td>0.295</td> <td>0.249</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 3</td> <td>0.271</td> <td>0.207</td> <td>0.078</td> <td>0.231</td> <td>0.239</td> <td>0.244</td> <td>0.302</td> <td>0.243</td> <td>0.041</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 4</td> <td>0.267</td> <td>0.192</td> <td>0.082</td> <td>0.229</td> <td>0.247</td> <td>0.237</td> <td>0.293</td> <td>0.257</td> <td>0.045</td>
| |
− | </tr>
| |
− | </table><br />
| |
− | <table border="1">
| |
− | <tr>
| |
− | <th>Hour 6:</th> <th>Neg. Control</th> <th>Pos. Control</th> <th>Device 1</th> <th>Device 2</th> <th>Device 3</th> <th>Device 4</th> <th>Device 5</th> <th>Device 6</th> <th>LB + Chlor (blank)</th>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 1</td> <td>0.358</td> <td>0.337</td> <td>0.168</td> <td>0.351</td> <td>0.318</td> <td>0.328</td> <td>0.332</td> <td>0.32</td> <td>0.044</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 2</td> <td>0.39</td> <td>0.359</td> <td>0.176</td> <td>0.358</td> <td>0.344</td> <td>0.35</td> <td>0.372</td> <td>0.34</td> <td>0.05</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 3</td> <td>0.399</td> <td>0.36</td> <td>0.177</td> <td>0.368</td> <td>0.347</td> <td>0.346</td> <td>0.359</td> <td>0.343</td> <td>0.045</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 1, Replicate 4</td> <td>0.402</td> <td>0.359</td> <td>0.177</td> <td>0.365</td> <td>0.346</td> <td>0.358</td> <td>0.364</td> <td>0.351</td> <td>0.054</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 1</td> <td>0.315</td> <td>0.277</td> <td>0.183</td> <td>0.322</td> <td>0.301</td> <td>0.348</td> <td>0.33</td> <td>0.32</td> <td>0.047</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 2</td> <td>0.329</td> <td>0.289</td> <td>0.186</td> <td>0.351</td> <td>0.319</td> <td>0.389</td> <td>0.353</td> <td>0.331</td> <td>0.051</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 3</td> <td>0.339</td> <td>0.301</td> <td>0.189</td> <td>0.358</td> <td>0.344</td> <td>0.371</td> <td>0.359</td> <td>0.362</td> <td>0.052</td>
| |
− | </tr>
| |
− | <tr>
| |
− | <td>Colony 2, Replicate 4</td> <td>0.337</td> <td>0.307</td> <td>0.202</td> <td>0.354</td> <td>0.331</td> <td>0.387</td> <td>0.363</td> <td>0.356</td> <td>0.076</td>
| |
− | </tr>
| |
− | </table><br />
| |
| <img src="https://static.igem.org/mediawiki/2017/b/b4/T--Jilin_China--interlab6.jpg" /><br /><br /> | | <img src="https://static.igem.org/mediawiki/2017/b/b4/T--Jilin_China--interlab6.jpg" /><br /><br /> |
| <img src="https://static.igem.org/mediawiki/2017/e/e8/T--Jilin_China--interlab7.jpg" /> | | <img src="https://static.igem.org/mediawiki/2017/e/e8/T--Jilin_China--interlab7.jpg" /> |