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<p><h2>Mentoring with UCCKE</h2></p> | <p><h2>Mentoring with UCCKE</h2></p> | ||
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> | ||
− | The Hong Kong UCCKE team aims to create biobricks that detect and control gout by | + | The Hong Kong UCCKE team aims to create biobricks that detect and degrade uric acid in order to control gout disease. We mentored the team by providing advice, protocols and also helping them to do experimental work. In the beginning of their project, they had difficulty in designing their experiment, especially when designing the assay for characterizing their biobricks. We helped them to construct an assay for measuring the fluorescence signal of their detection module. The assay can be done by incubating transformed E. coli in LB medium with saturated uric acid overnight followed by measuring fluorescent signal in plate reader. The detailed protocols can be viewed below. When they started doing their wet lab, they encountered difficulty in cloning their biobricks. To help them solve the problem, we have provided many advice. For example, to deal with the low insert concentration problem, we suggested them to amplify the synthesized DNA before doing restriction cut. We also suggested them to do restriction map before sequencing. We emphasized them the importance of negative and positive controls when troubleshooting problem of cloning and assaying. At the final stage of the project, we helped them to clone biobricks in parallel. We also helped them to sequence their plasmids and analyze sequencing result. Luckily, they constructed their biobricks on time. After having the biobricks, we helped them to perform the uric acid assay because they do not have plate reader in their school. In return, the Hong Kong UCCKE team helped us to 3D- print our hardware detection kit. They also provided us suggestions on the improvement of the prototype. The resulting product can be found in our prototype page. |
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− | In return, the Hong Kong UCCKE team | + | |
<img src="https://static.igem.org/mediawiki/2017/7/70/UCCKE1.jpg" width="450px" height="auto"><img src="https://static.igem.org/mediawiki/2017/b/b6/T--Hong_Kong_UCCKE--CU_MEETING_1.jpg" width="450px" height="auto"> | <img src="https://static.igem.org/mediawiki/2017/7/70/UCCKE1.jpg" width="450px" height="auto"><img src="https://static.igem.org/mediawiki/2017/b/b6/T--Hong_Kong_UCCKE--CU_MEETING_1.jpg" width="450px" height="auto"> |
Revision as of 15:06, 30 October 2017