Difference between revisions of "Team:SJTU-BioX-Shanghai/Part Collection"

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                     <a class="navbar-brand" href="https://2017.igem.org/Team:SJTU-BioX-Shanghai"><img src="https://static.igem.org/mediawiki/2017/b/be/T--SJTU-BioX-Shanghai--logo.png" height="55" /></a>
 
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                             <li class="nav-item"><a href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Model" class="nav-link">Model</a></li>
 
                             <li class="nav-item"><a href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Model" class="nav-link">Model</a></li>
 
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                             <li class="nav-item"><a href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Team" class="nav-link">Team</a></li>
 
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                         <div class="my-title h5-my-responsive">Introduction</div>
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                         <div class="my-title h5-my-responsive">STAR-Chromoprotein</div>
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                         <p>This year, our team will choose to present STAR 3, another modified STAR (Small Transcriptional-Activating RNA) for the award of the basic part (<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285020">BBa_K2285020</a>).
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                         <p>We chose parts we have built relevant to our multifactorial visualized detection system for this year’s parts collection.</p>
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                            Our project was based on iGEM16_Imperial STAR system (to distinguish it from our work, we called it <strong>STAR 1 system</strong>), and we decided to build another STAR system (we called it <strong>STAR 3 system</strong>)
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                        <p class="my-title2">How to design your own multifactorial visualized detection system?</p>
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                            to achieve multiple responds to different conditions. To put it simply, two STAR systems together can control two genetic circuits. For more information about advantages of STAR and how it works, you can be refer to <a target="_blank"
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                                href="https://2016.igem.org/Team:Imperial_College/Basic_Part">here</a> .</p>
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                             <img src="https://static.igem.org/mediawiki/2017/5/59/T--SJTU-BioX-Shanghai--17201.png" class="img-fluid">
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                             <div class="figure-text"><strong>Figure 1. The STAR construct. </strong>In the absence of antisense, sense target RNA will form a stem-loop structure, functioning as a terminator and stop the following transcription. When antisense RNA, a mRNA fragment
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                             <div class="figure-text"><strong>Figure 1. The STAR-chromoprotein construct.</strong></div>
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                                which is the antisense of part of the target RNA, is transcribed, the terminator structure will be disrupted and switch on the inhibited transcription.</div>
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                         </div>
 
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                        <p class="my-title2">For convenience, we want to introduce some terms for later description:</p>
 
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                        <p>In the original project of Imperial, the stem-loop structure, which could be seen as a terminator, on the upstream of functional genes is called STAR-Target and we would just name it <strong>Target</strong>. The DNA sequence that
 
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                            transcribes STAR we rename it <strong>Antisense</strong>. When we say <strong>Target 1</strong> and <strong>Antisense 1</strong>, they refer to the terminator structure and its complementary sequence in Imperial’s project.
 
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                            When we say <strong>Target 3</strong> and <strong>Antisense 3</strong>, they refer to the novel terminator structure and its complementary sequence we designed this year. Target 1 and Antisense 1 together constitute STAR 1
 
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                            system and Target 3 and Antisense 3 constitute STAR 3 system.</p>
 
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                        <p>Please visit our <a target="_blank" href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Design">Design page</a> to find more information about how we designed the STAR 3 system.</p>
 
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                        <p>Alternatively check out Target 3 <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285020">BBa_K2285020</a> webpage for characterization of the STARs (Both STAR 1 and STAR 3) system.</p>
 
  
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                         <div class="my-title h5-my-responsive">Basic Parts we submitted</div>
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                        <p>To illustrate, we have built two circuit to detect two heavy metal ions, As and Co. (For more information about how these circuits detect factors, see our <a target="_blank" href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Design">Design page</a>)
 +
                            What should be noticed is although we used heavy metal ions detection promoters, there are indeed many promoters you can used to control circuits such as promoter relative to light, cellular signaling construction and change
 +
                            of human body environment.</p>
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                        <div class="figure-intro">
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                            <img src="https://static.igem.org/mediawiki/2017/d/d4/T--SJTU-BioX-Shanghai--17203.png" class="img-fluid">
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                            <div class="figure-text"><strong>Figure 2. The STAR 3 - As - CP construct</strong></div>
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                        </div>
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                        <div class="figure-intro">
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                            <img src="https://static.igem.org/mediawiki/2017/f/f0/T--SJTU-BioX-Shanghai--17204.png" class="img-fluid">
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                            <div class="figure-text"><strong>Figure 3. The STAR 1 - Co - GFP construct</strong></div>
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                        </div>
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                         <div class="my-title h5-my-responsive">Table of Composite part we submitted</div>
 
                         <div class="figure-intro" style="margin-top: 0px;">
 
                         <div class="figure-intro" style="margin-top: 0px;">
 
                             <table class="table table-res table-res2 table-hover" align="center">
 
                             <table class="table table-res table-res2 table-hover" align="center">
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                                 <tbody>
 
                                 <tbody>
 
                                     <tr>
 
                                     <tr>
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                                         <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285010">BBa_K2285010</a></td>
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                                         <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285012">BBa_K2285012</a></td>
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                                         <td>RNA</td>
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                                         <td>Composite</td>
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                                         <td>Antisense3+t500</td>
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                                         <td>J23119+Target3+eforRed(with RBS and Term added)</td>
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                                         <td>104</td>
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                                        <td>894</td>
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                                    </tr>
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                                    <tr>
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                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285013">BBa_K2285013</a></td>
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                                        <td>Composite</td>
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                                        <td>J23119+Target3+amilGFP(with RBS and Term added)</td>
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                                        <td>912</td>
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                                    </tr>
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                                    <tr>
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                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285014">BBa_K2285014</a></td>
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                                        <td>Composite</td>
 +
                                        <td>J23119+Target3+cjBlue(with RBS and Term added)</td>
 +
                                        <td>915</td>
 +
                                    </tr>
 +
                                    <tr>
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                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285015">BBa_K2285015</a></td>
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                                        <td>Composite</td>
 +
                                        <td>J23119+Target3+aimlCP(with RBS and Term added)</td>
 +
                                        <td>960</td>
 +
                                    </tr>
 +
                                    <tr>
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                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285016">BBa_K2285016</a></td>
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                                        <td>Composite</td>
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                                        <td>J23119+Target1+eforRed(with RBS and Term added)</td>
 +
                                        <td>894</td>
 +
                                    </tr>
 +
                                    <tr>
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                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285017">BBa_K2285017</a></td>
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                                        <td>Composite</td>
 +
                                        <td>J23119+Target1+amilGFP(with RBS and Term added)</td>
 +
                                        <td>912</td>
 +
                                    </tr>
 +
                                    <tr>
 +
                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285018">BBa_K2285018</a></td>
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                                        <td>Composite</td>
 +
                                        <td>J23119+Target1+cjBlue(with RBS and Term added)</td>
 +
                                        <td>915</td>
 +
                                    </tr>
 +
                                    <tr>
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                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285019">BBa_K2285019</a></td>
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                                        <td>Composite</td>
 +
                                        <td>J23119+Target1+amilCP(with RBS and Term added)</td>
 +
                                        <td>960</td>
 +
                                    </tr>
 +
                                    <tr>
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                                        <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285024">BBa_K2285024</a></td>
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                                        <td>Composite</td>
 +
                                        <td>As promoter + Antisense3 + t500</td>
 +
                                         <td>630</td>
 
                                     </tr>
 
                                     </tr>
 
                                     <tr>
 
                                     <tr>
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                                         <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285020">BBa_K2285020</a></td>
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                                         <td><a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2285025">BBa_K2285025</a></td>
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                                         <td>Regulatory</td>
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                                         <td>Composite</td>
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                                         <td>J23119+Target3</td>
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                                         <td>Co promoter + Antisense1 + t5000</td>
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                                         <td>128</td>
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                                         <td>538</td>
 
                                     </tr>
 
                                     </tr>
 
                                 </tbody>
 
                                 </tbody>
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Revision as of 15:07, 30 October 2017

Part Collection
STAR-Chromoprotein

We chose parts we have built relevant to our multifactorial visualized detection system for this year’s parts collection.

How to design your own multifactorial visualized detection system?

Figure 1. The STAR-chromoprotein construct.

To illustrate, we have built two circuit to detect two heavy metal ions, As and Co. (For more information about how these circuits detect factors, see our Design page) What should be noticed is although we used heavy metal ions detection promoters, there are indeed many promoters you can used to control circuits such as promoter relative to light, cellular signaling construction and change of human body environment.

Figure 2. The STAR 3 - As - CP construct
Figure 3. The STAR 1 - Co - GFP construct
Table of Composite part we submitted
Part Number Type Description Length (bp)
BBa_K2285012 Composite J23119+Target3+eforRed(with RBS and Term added) 894
BBa_K2285013 Composite J23119+Target3+amilGFP(with RBS and Term added) 912
BBa_K2285014 Composite J23119+Target3+cjBlue(with RBS and Term added) 915
BBa_K2285015 Composite J23119+Target3+aimlCP(with RBS and Term added) 960
BBa_K2285016 Composite J23119+Target1+eforRed(with RBS and Term added) 894
BBa_K2285017 Composite J23119+Target1+amilGFP(with RBS and Term added) 912
BBa_K2285018 Composite J23119+Target1+cjBlue(with RBS and Term added) 915
BBa_K2285019 Composite J23119+Target1+amilCP(with RBS and Term added) 960
BBa_K2285024 Composite As promoter + Antisense3 + t500 630
BBa_K2285025 Composite Co promoter + Antisense1 + t5000 538
Reference
  1. Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs. Nat Chem Biol 11:214–220.
  2. Meyer, S., Chappell, J., Sankar, S., Chew, R., and Lucks, J. B. (2016) Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies Biotechnol. Bioeng. 113, 216.
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