Difference between revisions of "Team:BIT/part"
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Revision as of 16:14, 30 October 2017
| Biobrick | Name | Description | Length |
|---|---|---|---|
| Receptor expression | |||
| BBa_2368003 | T1R2 | Human Sweet-receptor | 2520 |
| BBa_2368004 | T1R3 | Human Sweet-receptor | 1989 |
| BBa_2368007 | PGal 1/10-T1R2-CYC1t | Completed expression of the human Sweet-receptor | 3449 |
| BBa_2368008 | PGal 1/10-T1R3-ADH1 | Completed expression of the human Sweet-receptor | 2814 |
| BBa_2368023 | MYC+T1R2 overlap | Marker genes of the Sweet-receptor | 349 |
| BBa_2368009 | HIS+T1R2 overlap | Marker genes of the Sweet-receptor | 50 |
| BBa_2368024 | HIS+T1R3 overlap | Marker genes of the Sweet-receptor | 187 |
| BBa_2368011 | FLAG+T1R3 overlap | Marker genes of the Sweet-receptor | 50 |
| BBa_2368012 | YCP+T1R2 overlap | Marker genes of the Sweet-receptor | 740 |
| BBa_2368013 | YCP+T1R3 overlap | Marker genes of the Sweet-receptor | 740 |
| BBa_2368014 | BCP+T1R2 overlap | Marker genes of the Sweet-receptor | 710 |
| BBa_2368015 | GFP+T1R2 overlap | Marker genes of the Sweet-receptor | 699 |
| BBa_2368020 | BCP+T1R3 overlap | Marker genes of the Sweet-receptor | 710 |
| Host engineering | |||
| BBa_2368017 | 500bp Homologous arm＋His | Knock out the sst2 gene | 2050 |
| BBa_2368018 | 500bp Homologous arm＋Ura | Knock out the far1 gene | 2050 |
| BBa_2368016 | 500bp Homologous arm＋Trp | Knock out the ste2 gene | 2071 |
| BBa_2368021 | Modified Gα Schizosaccharomyces pombe | Signal transduction | 1421 |
| BBa_2368022 | Modified Gα Caenorhabditis elegans | Signal transduction | 1418 |
| BBa_2368028 | Gα | Signal transduction | 1421 |
| Detection circuit | |||
| BBa_2368005 | Pfus | Induced promoter | 438 |
| BBa_2368026 | Pfus(4 STE12 binding sites) | Engineered promoter | 223 |
| BBa_2368027 | Pfus(6 STE12 binding sites) | Engineered promoter | 249 |
| BBa_2368006 | Pfus+mRFP+CYC1t | The reporter | 1375 |
| BBa_2368002 | CYC1t | A terminator in the yeast | 231 |
| BBa_2368029 | Pfus(2 STE12 binding sites) | Engineered promoter | 213 |
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