Difference between revisions of "Team:Linkoping Sweden/construct"

Line 12: Line 12:
 
<div class="personal_presentation_flip">
 
<div class="personal_presentation_flip">
 
                 <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
 
                 <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
−
<p> We designed one (super)plasmid that containd the genes for the three different cheperone systems we are useing. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone PSB1C3 for our (super)plasmid.   
+
<p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroEs, DnaK and trigger factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone PSB1C3 for our superplasmid.   
 
</p>
 
</p>
 
</div>
 
</div>
Line 19: Line 19:
 
<div class="personal_presentation_flip">
 
<div class="personal_presentation_flip">
 
                 <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
 
                 <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
−
<p> We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to M-neongreen protein.  
+
<p> We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to M-neongreen protein. For these plasmids we used an arabinose promotor. We put these substrates in the PSB1A3 backbone from iGEM. </p>
 +
 
 +
<H4> Tau </H4>
 +
<p> We designed one plasmid with Tau bound to GFP and one plasmid with Tau bound to M-neongeen protein. We used the arabinose promotor here.
 +
 
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 13:58, 28 June 2017

Our constructs

Chaperone systems

We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroEs, DnaK and trigger factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone PSB1C3 for our superplasmid.

Amyloid-beta

We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to M-neongreen protein. For these plasmids we used an arabinose promotor. We put these substrates in the PSB1A3 backbone from iGEM.

Tau

We designed one plasmid with Tau bound to GFP and one plasmid with Tau bound to M-neongeen protein. We used the arabinose promotor here.