<b>Design 4 (Modification of tablets package)</b><br>
<b>Design 4 (Modification of tablets package)</b><br>
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During the conversation with Prof. Sung, we inquired him the problems faced in the last prototype. He suggested adding the samples to small amount of ddH2O before transferring to the detector, the cell-free system and it can help stabilizing the RNA inside the samples.
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During the conversation with Prof. Sung, we inquired him the problems faced in the last prototype. He suggested adding the samples to small amount of PBS before transferring to the detector, the cell-free system and it can help stabilizing the RNA inside the samples.
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Therefore, new distribution and allocation of wells inside the detection plate are changed: For medical track, each user/patient will consume 2 plates, one is the detector with freeze-dried cell-free system, another is only plate with water for sample stabilization; For subtyping, the plate is advanced by the referral from medical experts by adding more subtype detection. According to the latest information from the Centers for Disease Control and Prevention<sup>3</sup> (lastly updated on 19 April 2017), there are 18 known HA subtypes and 11 known NA subtypes up to now and many different combinations of HA and NA proteins are possible. Thus, a plate with two rows, HA and NA with 31 wells in total (18 for HA, 11 for NA and 2 for controls, positive and negative).
Therefore, new distribution and allocation of wells inside the detection plate are changed: For medical track, each user/patient will consume 2 plates, one is the detector with freeze-dried cell-free system, another is only plate with water for sample stabilization; For subtyping, the plate is advanced by the referral from medical experts by adding more subtype detection. According to the latest information from the Centers for Disease Control and Prevention<sup>3</sup> (lastly updated on 19 April 2017), there are 18 known HA subtypes and 11 known NA subtypes up to now and many different combinations of HA and NA proteins are possible. Thus, a plate with two rows, HA and NA with 31 wells in total (18 for HA, 11 for NA and 2 for controls, positive and negative).