Difference between revisions of "Team:Linkoping Sweden/construct"

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<H4> Chaperone systems </H4>
 
<H4> Chaperone systems </H4>
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<p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and trigger factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid.
 
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</p>
 
 
<div class="personal_presentation_flip">
 
<div class="personal_presentation_flip">
 
                 <img id="superplasmid"src="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG"/>
 
                 <img id="superplasmid"src="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG"/>
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<p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and trigger factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid.
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</p>
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</div>
 
</div>
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            <div class="personal_presentation">
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                <img class="team_member" src="https://static.igem.org/mediawiki/2017/5/56/T--Linkoping_Sweden--Max.jpg"/>
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                <h4><i>Max Lindberg</i></h4>
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                <p>
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                <b>Education: </b>4th year of MSc in Protein Science<br>
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                <b>Groups: </b>Project leader<br>
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                <b>Why iGEM? </b>Because synthetic biology is f*cking awesome! :D
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                </p>
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            </div>
  
 
<H4> Amyloid-beta </H4>
 
<H4> Amyloid-beta </H4>

Revision as of 14:43, 28 June 2017

Our constructs

Chaperone systems

We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and trigger factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid.

Max Lindberg

Education: 4th year of MSc in Protein Science
Groups: Project leader
Why iGEM? Because synthetic biology is f*cking awesome! :D

Amyloid-beta

We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to mNeonGreen protein. For these plasmids we used an arabinose promotor. We put these substrates in the psb1A3 backbone from iGEM.

Tau

We designed one plasmid with Tau bound to GFP and one plasmid with Tau bound to mNeonGreen protein. We used the arabinose promotor and the psb1A3 backbone here as well.