Difference between revisions of "Team:Linkoping Sweden/construct"

Line 19: Line 19:
  
 
<H4> Amyloid-beta </H4>
 
<H4> Amyloid-beta </H4>
−
<p> We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to mNeonGreen protein. For these plasmids we used an arabinose promotor. We put these substrates in the psb1A3 backbone from iGEM. </p>
 
 
<div class="personal_presentation_flip">
 
<div class="personal_presentation_flip">
−
              <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
+
            <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
 +
<p> We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to mNeonGreen protein. For these plasmids we used an arabinose promotor. We put these substrates in the psb1A3 backbone from iGEM. </p>
 +
 
 +
</div>
 +
 
  
 
<H4> Tau </H4>
 
<H4> Tau </H4>
−
<p> We designed one plasmid with Tau bound to GFP and one plasmid with Tau bound to mNeonGreen protein. We used the arabinose promotor and the psb1A3 backbone here as well. </p>
 
 
<div class="personal_presentation_flip">
 
<div class="personal_presentation_flip">
 
                 <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
 
                 <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/>
 +
<p> We designed one plasmid with Tau bound to GFP and one plasmid with Tau bound to mNeonGreen protein. We used the arabinose promotor and the psb1A3 backbone here as well. </p>
 +
 +
</div>
 +
  
 
</div>
 
</div>

Revision as of 14:45, 28 June 2017

Our constructs

Chaperone systems

We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and trigger factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid.

Amyloid-beta

We designed one plasmids with amyloid-beta bound to GFP and one plasmid with Amyloid-beta bound to mNeonGreen protein. For these plasmids we used an arabinose promotor. We put these substrates in the psb1A3 backbone from iGEM.

Tau

We designed one plasmid with Tau bound to GFP and one plasmid with Tau bound to mNeonGreen protein. We used the arabinose promotor and the psb1A3 backbone here as well.