Difference between revisions of "Team:iTesla-SoundBio/Results"

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<h1>Results</h1>
 
<h1>Results</h1>
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<p>We ultimately sent in two different biobricks: the upstream region of genes pcbA4/5 (BBa_K2363001), and the pcbA5 gene itself (BBa_K2363000). Our control was the digested backbone vector without insert added in the ligation reaction. The plate showed several red colonies and one white colony. We expected nothing to show up, but hey stuff happens. Our plate containing our promoter biobrick had significant growth of white colonies, suggesting the ligations and transformations were effective, so we went on to miniprep and sequence four of the white colonies to make sure they weren’t false positives. There were a few red colonies on the plate which suggests that the digest wasn’t 100% effective and the RFP protein was still expressed. Our pcbA5 biobrick didn’t show as much growth, and there were more red colonies than white colonies. Nevertheless, we went on and miniprepped and sequenced two of the white colonies.</p>
  
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<p>Here you can describe the results of your project and your future plans. </p>
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<img src="https://static.igem.org/mediawiki/2017/3/3c/T--iTesla-SoundBio--results3.png" align="left" width="300px" /></p>
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<h5>What should this page contain?</h5>
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<p>Testing, testing, 1 2 ,3</p><br clear="all" />
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h5> Project Achievements </h5>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<p><img src="https://static.igem.org/mediawiki/2017/b/b7/T--iTesla-SoundBio--results1.png" align="left" width="300px" /></p>
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<p>ABC: Testing, testing, 1 2 ,3</p><br clear="all" />
  
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<p><img src="https://static.igem.org/mediawiki/2017/0/0b/T--iTesla-SoundBio--results2.png" align="left" width="300px" /></p>
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<p>123: Testing, testing, 1 2 ,3</p><br clear="all" />
  
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Sequencing revealed that both the promoter and pcbA5 biobricks obtained from our selected colonies perfectly matched our expected constructs. See raw data attached:
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<h5>Inspiration</h5>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Revision as of 02:34, 31 October 2017

Results

We ultimately sent in two different biobricks: the upstream region of genes pcbA4/5 (BBa_K2363001), and the pcbA5 gene itself (BBa_K2363000). Our control was the digested backbone vector without insert added in the ligation reaction. The plate showed several red colonies and one white colony. We expected nothing to show up, but hey stuff happens. Our plate containing our promoter biobrick had significant growth of white colonies, suggesting the ligations and transformations were effective, so we went on to miniprep and sequence four of the white colonies to make sure they weren’t false positives. There were a few red colonies on the plate which suggests that the digest wasn’t 100% effective and the RFP protein was still expressed. Our pcbA5 biobrick didn’t show as much growth, and there were more red colonies than white colonies. Nevertheless, we went on and miniprepped and sequenced two of the white colonies.

Testing, testing, 1 2 ,3


ABC: Testing, testing, 1 2 ,3


123: Testing, testing, 1 2 ,3




Sequencing revealed that both the promoter and pcbA5 biobricks obtained from our selected colonies perfectly matched our expected constructs. See raw data attached: