Difference between revisions of "Team:Kyoto/Basic Part"

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   <h3><Div Align="center">Rev Protein</Div></h3>
 
   <h3><Div Align="center">Rev Protein</Div></h3>
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   <p>For this year’s iGEM competition, we have chosen to present Rev protein for the award of the basic  part(BBa_K2403000). In synthetic biology, RNA which has a complex structure is frequently designed and used. If such RNA is transferred in Eukaryotic cell, Tap/p15 cannot transfer normal single mRNA to cytoplasm, and the RNA can remain in the nucleus. We introduced Rev protein and RRE (rev response element) to transfer such artificial RNA to the cytoplasm. iGEMers can transport the RNA to the cytoplasm if they bind RRE to any RNA and express Rev protein simultaneously. </p>
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   <p>For this year’s iGEM competition, we have chosen to submit Rev protein for the award of the Best Basic part (BBa_K2403000). In eukaryotic cells mature mRNA is exported by Tap/p15 (Mex67p/ Mtr2p in yeast) from the nucleus into the cytoplasm for translation into protein. However, in synthetic biology, other types of RNA with complex structures are frequently designed and used. If RNA with complex structure is expressed in a eukaryotic cell, Tap/p15 cannot export it effectively, and the RNA will remain in the nucleus. To solve this problem, we developed parts for the HIV Rev protein and associated cis-acting RRE (rev response element) for nuclear export of artificial RNA. iGEMers can export their RNA sequences containing an RRE to the cytoplasm if they express Rev protein simultaneously. </p>
 
    
 
    
 
   <h3><Div Align="center">How Rev works</Div></h3>
 
   <h3><Div Align="center">How Rev works</Div></h3>
  
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   <P>Rev protein binds to target sequence, RRE. CRM1, a principal nuclear export factor in cells, is RnaGTP-dependent, binds to Rev protein, and transfers Ribonucleoprotein(RNP) to the extranuclear.</p>
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   <p>Rev proteins bind to RRE sequences engineered into target RNAs. Rev recruits CRM1, a principal nuclear export factor in cells which mediates export of cellular RNAs including snRNA and 5S rRNA to the cytoplasm.</p>
  
 
   <h3><Div Align="center">Key advantage</Div></h3>
 
   <h3><Div Align="center">Key advantage</Div></h3>
 
    
 
    
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   <p>Rev protein makes it possible to transport RNA to the extranuclear which is needed but can’t be normally.</p>
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   <p>Rev protein makes it possible to export complex RNA structures from the nucleus to the cytoplasm, which otherwise cannot be achieved effectively.</p>
 
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Revision as of 03:36, 31 October 2017

Basic Part

Rev Protein

For this year’s iGEM competition, we have chosen to submit Rev protein for the award of the Best Basic part (BBa_K2403000). In eukaryotic cells mature mRNA is exported by Tap/p15 (Mex67p/ Mtr2p in yeast) from the nucleus into the cytoplasm for translation into protein. However, in synthetic biology, other types of RNA with complex structures are frequently designed and used. If RNA with complex structure is expressed in a eukaryotic cell, Tap/p15 cannot export it effectively, and the RNA will remain in the nucleus. To solve this problem, we developed parts for the HIV Rev protein and associated cis-acting RRE (rev response element) for nuclear export of artificial RNA. iGEMers can export their RNA sequences containing an RRE to the cytoplasm if they express Rev protein simultaneously.

How Rev works

Rev proteins bind to RRE sequences engineered into target RNAs. Rev recruits CRM1, a principal nuclear export factor in cells which mediates export of cellular RNAs including snRNA and 5S rRNA to the cytoplasm.

Key advantage

Rev protein makes it possible to export complex RNA structures from the nucleus to the cytoplasm, which otherwise cannot be achieved effectively.