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<p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. | <p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. | ||
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+ | <p> For the GroEL/GroEs chaperone system we used the T7 promotor which is induced by addition of IPTG. | ||
</p> | </p> | ||
Revision as of 11:07, 29 June 2017