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<p> For the GroEL/GroEs chaperone system we used the T7 promotor which is induced by addition of IPTG. | <p> For the GroEL/GroEs chaperone system we used the T7 promotor which is induced by addition of IPTG. | ||
− | <br> For the Trigger Factor gene, we chose a tetracycline promotor, therefor we also had to put in the gene for tetracycline resistance. | + | <br>For the Trigger Factor gene, we chose a tetracycline promotor, therefor we also had to put in the gene for tetracycline resistance. |
− | + | <br> | |
<br> The tetracycline promotor is induced by adding tetracycline. | <br> The tetracycline promotor is induced by adding tetracycline. | ||
The last promotor we used in the superduper-plasmid was a rhamnose promotor called pRha, this promotor was used for the DnaK gene and is induced by adding L-rhamnose. | The last promotor we used in the superduper-plasmid was a rhamnose promotor called pRha, this promotor was used for the DnaK gene and is induced by adding L-rhamnose. |
Revision as of 11:23, 29 June 2017