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<p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. | <p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. | ||
− | <p> For the GroEL/GroEs chaperone system we used the T7 promotor which is induced by addition of IPTG. | + | <p> For the GroEL/GroEs chaperone system we used the T7 promotor which is induced by addition of IPTG. </p> |
− | + | <p> For the Trigger Factor gene, we chose a tetracycline promotor, therefor we also had to put in the gene for tetracycline resistance. </p> | |
− | < | + | <p>The tetracycline promotor is induced by adding tetracycline. |
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The last promotor we used in the superduper-plasmid was a rhamnose promotor called pRha, this promotor was used for the DnaK gene and is induced by adding L-rhamnose. | The last promotor we used in the superduper-plasmid was a rhamnose promotor called pRha, this promotor was used for the DnaK gene and is induced by adding L-rhamnose. | ||
Revision as of 11:24, 29 June 2017