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<img id="superplasmid"src="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG"/> | <img id="superplasmid"src="https://static.igem.org/mediawiki/2017/b/ba/T--Linkoping_Sweden--superduperplasmid.PNG"/> | ||
</a> | </a> | ||
− | <p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. | + | <p> We designed one superduper-plasmid with the genes for all three cheperone systems we are useing, GroEL/GroES, DnaK and Trigger Factor. We put in different promotors for these genes to be able to regulate the expression. We used the the iGEM backbone psb1C3 for our superplasmid. The psb1C3 backbone contains a gene for chloramphenicol resistence. |
<p> For the GroEL/GroEs chaperone system we used the T7 promotor which is induced by addition of IPTG. </p> | <p> For the GroEL/GroEs chaperone system we used the T7 promotor which is induced by addition of IPTG. </p> | ||
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<div class="personal_presentation_flip"> | <div class="personal_presentation_flip"> | ||
<img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/> | <img id="liu_igem" src="https://drive.google.com/uc?id=0B2_M7x6NmgaCM0tNZGM2QjB5dGM"/> | ||
− | <p> We designed one plasmids with amyloid-beta bound to | + | <p> We designed one plasmids with amyloid-beta bound to EGFP and one plasmid with Amyloid-beta bound to mNeonGreen protein. For these plasmids we used an arabinose promotor called AraC which is induced by addition of arabinos. We put these substrates in the psb1A3 backbone from iGEM. </p> |
</div> | </div> |
Revision as of 11:29, 29 June 2017