Difference between revisions of "Team:XJTLU-CHINA/Protocols Ligation"
(Created page with "{{XJTLU-CHINA}} <html> <head> <title>Ligation</title> <style> p{ font-size:18px !important; } .form{ font-size:16px !important; margi...") |
|||
| Line 4: | Line 4: | ||
<title>Ligation</title> | <title>Ligation</title> | ||
<style> | <style> | ||
| − | p{ | + | p, ol{ |
| − | font-size: | + | font-size:20px !important; |
} | } | ||
.form{ | .form{ | ||
Latest revision as of 08:14, 31 October 2017
Ligation
Procedure:
- Add 2μl digested plasmid backbone (25ng).
- Add equimolar digested fragment.
- Add 1μl T4 DNA ligase buffer.
- Add 0.5μl T4 DNA ligase.
- Add ddH2O to 10ul volume.
- Ligate at 16℃ for 30 minutes. Heat kill at 80℃ for 20 minutes.
- Transform 1-2μl of the product.
Collaborators and Supporters
