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Latest revision as of 08:37, 31 October 2017
Autolysis test
(Transformant with pNZ8148 harboring BBa_2309004):
- Grow 5 ml successfully transformed bacteria culture overnight, 30 °C.
- Dilute 5-fold with fresh medium.
- Aliquot 200μl culture into each well of 96-well plate. Grow at 30℃ in plate reader with a regular test every 30 minutes until stationary phase (OD600≥3).
- Induce with 0.5-5 ng/ml nisin (usually 1 ng/ml). Continue to observe the growth curve with a regular OD600 test every 1 hour.
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