Difference between revisions of "Team:XJTLU-CHINA/Protocols CFU measurement"
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Latest revision as of 08:37, 31 October 2017
Pre-experiment: CFU measurement
Reagents:
Staphylococcus aureus solution (37℃, 200rpm, overnight culture); LB agar plates
Procedure:
- Dilute Staphylococcus aureus solution with x10, x100, x500, x1000 concentration gradients. Vortex each concentration of solutions for 1-3min;
- Use microscope to count the cell number of different concentration gradient.
- Culture each concentration gradient of Staphylococcus aureus solution on LB agar. Add 20μl of solution on plate for each concentration. Do 3 repeats for each gradient.
- Overnight culture at 37℃ condition.
- After overnight culture, count colonies.
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