Difference between revisions of "Team:Jilin China/Basic Part"

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<div class="banner"><div class="menu">Basic Parts</div><img src="https://static.igem.org/mediawiki/2017/b/b1/T--Jilin_China--_sec_bg_t.jpg"></div>
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    <strong style="color: #229d73;">1.wt-Pr</strong> <br />
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<p>wt-Pr is a constitutive promoter which is in the upstream of DmpR, a transcriptional factor of dmp operon from Pseudomonas sp. Strain CF600 encoded by dmpR gene.[1]</p>
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<strong style="color: #229d73;">2.cphA-1</strong> <br />
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<p>CphA-1, a catechol 1,2-dioxygenase from Arthrobacter chlorophenolicus A6, is responsible for ring cleavage in aromatic compounds degrading process [2].</p>
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<div class="pic_box center">
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<img src="https://static.igem.org/mediawiki/2017/3/3e/T--Jilin_China--basic_parts01.png"  /><br />
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Figure 2. Reaction of cphA-1
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<strong style="color: #229d73;">3.CaO19</strong> <br />
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<p>CaO19, a hydroxyquinol 1,2-dioxygenase from Candida albicans TL3, is responsible for ring cleavage in aromatic compounds degrading process[3].</p>
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<div class="pic_box center">
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<img src="https://static.igem.org/mediawiki/2017/5/52/T--Jilin_China--basic_parts02.png"  /><br />
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Figure 3. Reaction of CaO19
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<strong style="color: #229d73;">4.CbtA</strong> <br />
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<p>CbtA is a protein found in crytic prophage CP4-44 in Escherichia coli K-12. CbtA could inhibit cell division and cell elongation via direct and independent interactions with FtsZ and MreB[4], so it is defined as a kind of toxin.</p>
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<strong style="color: #229d73;">5.CbeA </strong> <br />
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<p>CbeA is a protein found in crytic prophage CP4-44 in Escherichia coli K-12 which could suppress the effect of CbtA, so it is defined as a kind of antitoxin. Instead of interacting with CbtA, CbeA directly binds MreB and FtsZ and promotes the assembly of FtsZ and MreB filaments[4].</p>
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    <div class="pic_box center">
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<img src="https://static.igem.org/mediawiki/2017/9/91/T--Jilin_China--basic_parts03.png"  /><br />
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Figure 4. TA system
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<p>
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This year we use CbtA and CbeA to build the Geneguard system in our project. For detailed information about toxin/antitoxin (TA) system, <a href="https://2017.igem.org/Team:Jilin_China/Description">please visit ...</a>
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</p>
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<strong>Reference：</strong>
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<p>[1] Shingler, V., M. Bartilson, and T. Moore. Cloning and nucleotide sequence of the gene encoding the positive regulator (DmpR) of the phenol catabolic pathway encoded by pVI150 and identification of DmpR as a member of the NtrC family of transcriptional activators. J. Bacteriol. ( 1993) 175: 1596–1604.</p>
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<p>[2] Seok H. Lee. Effective biochemical decomposition of chlorinated aromatic hydrocarbons with a biocatalyst immobilized on a natural enzyme support. Bioresource Technology. (2013) 141:89–96.</p>
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<p>[3] Purification and characterization of a catechol 1,2-dioxygenase from a phenol degrading Candida albicans TL3. San-Chin Tsai · Yaw-Kuen Li Arch Microbiol. (2007) 187:199–206.</p>
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<p>[4] Masuda, Tan. YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli. Molecular Microbiology. (2012) 84(5), 979–989.</p>
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<h1>Basic Parts</h1>
 
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
 
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<h3>Best Basic Part Special Prize</h3>
 
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
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<h4>Note</h4>
 
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
 
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Revision as of 10:24, 31 October 2017

1.wt-Pr

wt-Pr is a constitutive promoter which is in the upstream of DmpR, a transcriptional factor of dmp operon from Pseudomonas sp. Strain CF600 encoded by dmpR gene.[1]


2.cphA-1

CphA-1, a catechol 1,2-dioxygenase from Arthrobacter chlorophenolicus A6, is responsible for ring cleavage in aromatic compounds degrading process [2].


Figure 2. Reaction of cphA-1

3.CaO19

CaO19, a hydroxyquinol 1,2-dioxygenase from Candida albicans TL3, is responsible for ring cleavage in aromatic compounds degrading process[3].


Figure 3. Reaction of CaO19

4.CbtA

CbtA is a protein found in crytic prophage CP4-44 in Escherichia coli K-12. CbtA could inhibit cell division and cell elongation via direct and independent interactions with FtsZ and MreB[4], so it is defined as a kind of toxin.


5.CbeA

CbeA is a protein found in crytic prophage CP4-44 in Escherichia coli K-12 which could suppress the effect of CbtA, so it is defined as a kind of antitoxin. Instead of interacting with CbtA, CbeA directly binds MreB and FtsZ and promotes the assembly of FtsZ and MreB filaments[4].


Figure 4. TA system

This year we use CbtA and CbeA to build the Geneguard system in our project. For detailed information about toxin/antitoxin (TA) system, please visit ...

Reference：

[1] Shingler, V., M. Bartilson, and T. Moore. Cloning and nucleotide sequence of the gene encoding the positive regulator (DmpR) of the phenol catabolic pathway encoded by pVI150 and identification of DmpR as a member of the NtrC family of transcriptional activators. J. Bacteriol. ( 1993) 175: 1596–1604.

[2] Seok H. Lee. Effective biochemical decomposition of chlorinated aromatic hydrocarbons with a biocatalyst immobilized on a natural enzyme support. Bioresource Technology. (2013) 141:89–96.

[3] Purification and characterization of a catechol 1,2-dioxygenase from a phenol degrading Candida albicans TL3. San-Chin Tsai · Yaw-Kuen Li Arch Microbiol. (2007) 187:199–206.

[4] Masuda, Tan. YeeU enhances the bundling of cytoskeletal polymers of MreB and FtsZ, antagonizing the CbtA (YeeV) toxicity in Escherichia coli. Molecular Microbiology. (2012) 84(5), 979–989.