Line 2: | Line 2: | ||
<html> | <html> | ||
− | <div class=" | + | <div class="banner"><div class="menu">Improvement</div><img src="https://static.igem.org/mediawiki/2017/b/b1/T--Jilin_China--_sec_bg_t.jpg"></div> |
− | + | ||
− | <div class=" | + | <div class="sec_box"> |
− | < | + | |
− | <p> | + | <div class="h1_title">1.Improvement in dector method in DmpR’s response intensity.</div> |
− | + | <div class="h1_content"> | |
− | < | + | <p>(1) We realize the quantitative measurement in DmpR’s response intensity using Reallia Luciferase. Compared to GFP <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/DmpR">team Peking-2013</a> use, we can detect DmpR’s inducing strength more accurately. |
− | <p>< | + | <div class="pic_box center"> |
− | + | <img src="https://static.igem.org/mediawiki/2017/4/4f/T--Jilin_China--improvement01.png" /><br /> | |
− | < | + | <img src="https://static.igem.org/mediawiki/2017/1/1d/T--Jilin_China--improvement02.png" /><br /> |
− | < | + | Figure 1. DmpR’s circuit. (A) Peking-2013 (B) Jilin-2017 |
− | + | </div> | |
− | + | </p> | |
− | </ | + | <p>(2) Renilla Luciferase enable us conduct the measurement in vivo, which provide a easier and faster method in detecting.</p> |
+ | </div> | ||
+ | |||
+ | <div class="h1_title">2.Improvement in response intensity toward certain phenolics.</div> | ||
+ | <div class="h1_content"> | ||
+ | <p>Team Jilin China in 2017 do several mutagenesis in sensor domain of DmpR. To compare the difference between <a href="https://2013.igem.org/Team:Peking/Project/BioSensors/DmpR">Peking-2013 DmpR</a> and our mutated DmpR downstream of the same constitutive promoter, we chose phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol as the inducer. Expression level of Renilla Luciferase downstream of dmp operon (P0 promoter) was used to indicate the response intensity towards different phenolics. Comparisons are based on ratio of different DmpR’s response and mock’s toward phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol respectively. | ||
+ | <div class="pic_box center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/be/T--Jilin_China--improvement03.png" /><br /> | ||
+ | Figure 1. The device we constructed to do comparison between peking-2013 dmpR and our mutated DmpR | ||
+ | </div> | ||
+ | </p><br /> | ||
+ | <p> | ||
+ | It turned out that both DmpR can response to phenol, 2-CP, pyrocatechol. However, compared to Peking-DmpR, our DmR showed better response to phenol and pyrocatechol. | ||
+ | <div class="pic_box center"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/7/7c/T--Jilin_China--improvement04.png" /><br /> | ||
+ | Figure 2. DmpR’s response sensitivity toward different phenolics. Jilin-2017-DmpR showed better response to phenol and pyrocatechol. | ||
+ | </div> | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | <script> | ||
+ | $(function() { | ||
+ | function DirectoryNav($h,config){ | ||
+ | this.opts = $.extend(true,{ | ||
+ | scrollThreshold:0.5, | ||
+ | scrollSpeed:700, | ||
+ | scrollTopBorder:400, | ||
+ | easing: 'swing', | ||
+ | delayDetection:200, | ||
+ | scrollChange:function(){} | ||
+ | },config); | ||
+ | this.$win = $(window); | ||
+ | this.$h = $h; | ||
+ | this.$pageNavList = ""; | ||
+ | this.$pageNavListLis =""; | ||
+ | this.$curTag = ""; | ||
+ | this.$pageNavListLiH = ""; | ||
+ | this.offArr = []; | ||
+ | this.curIndex = 0; | ||
+ | this.scrollIng = false; | ||
+ | this.init(); | ||
+ | } | ||
+ | DirectoryNav.prototype = { | ||
+ | init:function(){ | ||
+ | this.make(); | ||
+ | var Thisss = this; | ||
+ | setInterval(function(){ | ||
+ | Thisss.offArr = []; | ||
+ | Thisss.$h.each(function(){ | ||
+ | var $This = $(this), | ||
+ | offT = Math.round($This.offset().top); | ||
+ | Thisss.offArr.push(offT-200); | ||
+ | }); | ||
+ | },1000); | ||
+ | this.bindEvent(); | ||
+ | }, | ||
+ | make:function(){ | ||
+ | $("body").append('<div class="directory-nav" id="directoryNav"><ul></ul><span class="cur-tag"></span><span class="c-top"></span><span class="c-bottom"></span><span class="line"></span></div>'); | ||
+ | var $hs = this.$h, | ||
+ | $directoryNav = $("#directoryNav"), | ||
+ | temp = [], | ||
+ | index1 = 0, | ||
+ | index2 = 0; | ||
+ | $hs.each(function(index){ | ||
+ | var $this = $(this), | ||
+ | text = $this.text(); | ||
+ | if(!$this.hasClass("h2_title")){ | ||
+ | index1++; | ||
+ | if(index1%2==0) index2 = 0; | ||
+ | temp.push('<li class="l1"><span class="c-dot"></span><a class="l1-text">'+text+'</a></li>'); | ||
+ | }else{ | ||
+ | index2++; | ||
+ | temp.push('<li class="l2"><a class="l2-text">'+text+'</a></li>'); | ||
+ | } | ||
+ | }); | ||
+ | $directoryNav.find("ul").html(temp.join("")); | ||
+ | this.$pageNavList = $directoryNav; | ||
+ | this.$pageNavListLis = this.$pageNavList.find("li"); | ||
+ | this.$curTag = this.$pageNavList.find(".cur-tag"); | ||
+ | this.$pageNavListLiH = this.$pageNavListLis.eq(0).height(); | ||
+ | if(!this.opts.scrollTopBorder){ | ||
+ | this.$pageNavList.show(); | ||
+ | } | ||
+ | }, | ||
+ | posTag:function(top){ | ||
+ | this.$curTag.css({top:top+'px'}); | ||
+ | }, | ||
+ | ifPos:function(st){ | ||
+ | var offArr = this.offArr; | ||
+ | var windowHeight = Math.round(this.$win.height()); | ||
+ | for(var i=0;i<offArr.length;i++){ | ||
+ | if((offArr[i] - windowHeight + 650) < st) { | ||
+ | var $curLi = this.$pageNavListLis.eq(i), | ||
+ | tagTop = $curLi.position().top; | ||
+ | $curLi.addClass("cur").siblings("li").removeClass("cur"); | ||
+ | if($curLi.hasClass("l2")){ | ||
+ | this.$curTag.addClass("cur2"); | ||
+ | }else{ | ||
+ | this.$curTag.removeClass("cur2"); | ||
+ | } | ||
+ | this.curIndex = i; | ||
+ | this.posTag(tagTop-6); | ||
+ | this.opts.scrollChange.call(this); | ||
+ | } | ||
+ | } | ||
+ | }, | ||
+ | bindEvent:function(){ | ||
+ | var This = this, | ||
+ | show = false, | ||
+ | timer = 0; | ||
+ | this.$win.on("scroll",function(){ | ||
+ | var $this = $(this); | ||
+ | clearTimeout(timer); | ||
+ | timer = setTimeout(function(){ | ||
+ | This.scrollIng = true; | ||
+ | if($this.scrollTop()>This.opts.scrollTopBorder){ | ||
+ | if(!This.$pageNavListLiH) This.$pageNavListLiH = This.$pageNavListLis.eq(0).height(); | ||
+ | if(!show){ | ||
+ | This.$pageNavList.fadeIn(); | ||
+ | show = true; | ||
+ | } | ||
+ | This.ifPos( $(this).scrollTop() ); | ||
+ | }else{ | ||
+ | if(show){ | ||
+ | This.$pageNavList.fadeOut(); | ||
+ | show = false; | ||
+ | } | ||
+ | } | ||
+ | },This.opts.delayDetection); | ||
+ | }); | ||
+ | this.$pageNavList.on("click","li",function(){ | ||
+ | var $this = $(this), | ||
+ | index = $this.index(); | ||
+ | This.scrollTo(This.offArr[index]); | ||
+ | }) | ||
+ | }, | ||
+ | scrollTo: function(offset,callback) { | ||
+ | var This = this; | ||
+ | $('html,body').animate({ | ||
+ | scrollTop: offset | ||
+ | }, this.opts.scrollSpeed, this.opts.easing, function(){ | ||
+ | This.scrollIng = false; | ||
+ | if(callback){ | ||
+ | if(this.tagName.toLowerCase()=='body'){ | ||
+ | callback(); | ||
+ | } | ||
+ | } | ||
+ | }); | ||
+ | } | ||
+ | }; | ||
+ | var directoryNav = new DirectoryNav($(".h1_title,.h2_title"),{ | ||
+ | scrollTopBorder:400 | ||
+ | }); | ||
+ | }); | ||
+ | </script> | ||
</html> | </html> | ||
+ | {{Jilin_footer}} |
Revision as of 10:46, 31 October 2017
(1) We realize the quantitative measurement in DmpR’s response intensity using Reallia Luciferase. Compared to GFP team Peking-2013 use, we can detect DmpR’s inducing strength more accurately.
Figure 1. DmpR’s circuit. (A) Peking-2013 (B) Jilin-2017
(2) Renilla Luciferase enable us conduct the measurement in vivo, which provide a easier and faster method in detecting.
Team Jilin China in 2017 do several mutagenesis in sensor domain of DmpR. To compare the difference between Peking-2013 DmpR and our mutated DmpR downstream of the same constitutive promoter, we chose phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol as the inducer. Expression level of Renilla Luciferase downstream of dmp operon (P0 promoter) was used to indicate the response intensity towards different phenolics. Comparisons are based on ratio of different DmpR’s response and mock’s toward phenol, 2-CP, 4-CP, 2,4-DCP and pyrocatechol respectively.
Figure 1. The device we constructed to do comparison between peking-2013 dmpR and our mutated DmpR
It turned out that both DmpR can response to phenol, 2-CP, pyrocatechol. However, compared to Peking-DmpR, our DmR showed better response to phenol and pyrocatechol.
Figure 2. DmpR’s response sensitivity toward different phenolics. Jilin-2017-DmpR showed better response to phenol and pyrocatechol.