Difference between revisions of "Team:NUS Singapore/Overview"

Line 169: Line 169:
 
<div class="columnleft">
 
<div class="columnleft">
 
         <h2>Methodology</h2>
 
         <h2>Methodology</h2>
−
<p>With the advancement in the field of synthetic biology, scientists have successfully engineered microbes to combat challenges in various fields such as medicine, energy, and environment. These genetically engineered microbes are however not commercialized due to the risk of bacteria escaping from the targeted host into the environment. To address this issue, we develop the NUSgem Kill-switch Toolkit that enables users to build customized and effective kill-switches for different applications. This toolkit consists of a library of characterized sensors, logic gates, and killing systems. </p>
+
<p>Methodology describes the modelling process we use to develop each of our models. Here you can find information about the equations and modelling workflow we use.</p>
 
</div>
 
</div>
  
Line 179: Line 179:
  
 
<div class="columnleft">
 
<div class="columnleft">
−
       <h2>Kill-switch for probiotics</h2>
+
       <h2>Kill switch for probiotics</h2>
−
<p>With the advancement in the field of synthetic biology, scientists have successfully engineered microbes to combat challenges in various fields such as medicine, energy, and environment. These genetically engineered microbes are however not commercialized due to the risk of bacteria escaping from the targeted host into the environment. To address this issue, we develop the NUSgem Kill-switch Toolkit that enables users to build customized and effective kill-switches for different applications. This toolkit consists of a library of characterized sensors, logic gates, and killing systems. </p>
+
<p>The kill switch for probiotics is a successful proof of concept that demonstrates how our E2 chassis and modelling workflow can be used to make  the engineering of customised killswitches for engineered probiotics easier. Using this model, our experimenters successfuly constructed the phosphate-temperature cascaded system with GFP reporter <a href="http://parts.igem.org/Part:BBa_K2447015">(BBa_K2447015)</a>. Here you can find the modelling workflow and modelling results that proves our design works!</p>
 
</div>
 
</div>
  
Line 190: Line 190:
  
 
<div class="columnleft">
 
<div class="columnleft">
−
         <h2>Kill-switch for BeeT</h2>
+
         <h2>Kill switch for BeeT</h2>
−
<p>With the advancement in the field of synthetic biology, scientists have successfully engineered microbes to combat challenges in various fields such as medicine, energy, and environment. These genetically engineered microbes are however not commercialized due to the risk of bacteria escaping from the targeted host into the environment. To address this issue, we develop the NUSgem Kill-switch Toolkit that enables users to build customized and effective kill-switches for different applications. This toolkit consists of a library of characterized sensors, logic gates, and killing systems. </p>
+
<p>The kill switch for BeeT employs our E2 Chassis and automated circuit design included in our modelling workflow, to Wageningen iGEM2016's biocontainment system. We make improvements to their kill switch by increasing the kill switch's specificity, increasing  efficiency, and employing a more effective killing mechanism using our chassis.</p>
 
</div>
 
</div>
  

Revision as of 12:24, 31 October 2017

Measurement Modelling Medal Check List
Team Member Attributions
Collaboration
Silver Up Gold Integrated
Overview Methodology Kill Switch for Probiotics Kill Switch for BeeT
Description Interlab Study Parts Experiment Documentation Improvement Demonstrate Safety Future Works
Home

Overview

Methodology

Methodology describes the modelling process we use to develop each of our models. Here you can find information about the equations and modelling workflow we use.

Kill switch for probiotics

The kill switch for probiotics is a successful proof of concept that demonstrates how our E2 chassis and modelling workflow can be used to make the engineering of customised killswitches for engineered probiotics easier. Using this model, our experimenters successfuly constructed the phosphate-temperature cascaded system with GFP reporter (BBa_K2447015). Here you can find the modelling workflow and modelling results that proves our design works!

Kill switch for BeeT

The kill switch for BeeT employs our E2 Chassis and automated circuit design included in our modelling workflow, to Wageningen iGEM2016's biocontainment system. We make improvements to their kill switch by increasing the kill switch's specificity, increasing efficiency, and employing a more effective killing mechanism using our chassis.