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Revision as of 12:26, 31 October 2017
Model
Content
To estimate how our product will function the best, we analyzed the data we got from the experiments. First of all, conducting the glucose up take experiment, not only did we find the relation between time and the percentage of glucose absorbance, but proved that the engineered E.coli work perfectly. Additionally, we also carried on an examination to see whether the acid-resistance capsule containing bacteria dissolves in alkaline environment, representing intestine. With the results we got from the above experiments, we can tell how much probiotic consumers should take depending on the amount of sugar contained in the food.
As the figure shown below, we can see that the E.coli with PTS transporter had better performance on glucose absorbance. By the time of 4 hour, 1 ml of bacteria can absorb approximately 3.3 mg of glucose per hour.
With the data from the experiment, we can calculate how much glucose can a gram of probiotic absorb:
The average time food being absorbed in small intestine is about 5 hours, which means each gram of E.coli we engineered could absorb 2g of glucose when in small intestine.
According to the Ministry of Health and Welfare of the Republic of China, people can calculate how much calorie should one get every day (depending on BMI) with the formula below.
According to World Health Organization (WHO), the amount of sugar one consume should be less than 5% of recommended calorie each day. Then, we can calculate how much sugar does the bacteria need to absorb when having different kinds of food and drinks. The following figure shows how much bacteria should one take depending on the food or drink.
© iGEM Mingdao 2017. Design: Kevin Li. All rights reserved.
