Difference between revisions of "Team:Bristol/Results"
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| − | <h2>PCR results</h2> | + | <h2 class="featurette-heading Up">PCR results</h2> |
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The following table shows the final conditions used for successful PCR: | The following table shows the final conditions used for successful PCR: | ||
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| − | <h2>Sequencing results</h2> | + | <h2 class="featurette-heading Up">Sequencing results</h2> |
<p class="lead Up"> | <p class="lead Up"> | ||
We successfully performed PCR on our fragments with our editing primers, to isolate the whole coding regions. Sequencing of our constructs showed that most had significant deletions and insertions of unknown DNA, however one construct, NrfB pSB1C3, had | We successfully performed PCR on our fragments with our editing primers, to isolate the whole coding regions. Sequencing of our constructs showed that most had significant deletions and insertions of unknown DNA, however one construct, NrfB pSB1C3, had | ||
| − | only one point mutation (shown below). We submitted this part (see our <a target="_blank"href="https://2017.igem.org/Team:Bristol/Parts#part">Parts</a> page), annotating the mutation so future teams can take it into consideration when ordering the part. The part can still be used accurately if this mutation is edited, either molecularly, | + | only one point mutation (shown below). We submitted this part (see our <a target="_blank" href="https://2017.igem.org/Team:Bristol/Parts#part">Parts</a> page), annotating the mutation so future teams can take it into consideration when |
| − | + | ordering the part. The part can still be used accurately if this mutation is edited, either molecularly, or through DNA synthesis. | |
</p> | </p> | ||
| − | <img class="centreimage" src="https://static.igem.org/mediawiki/2017/5/5d/T--Bristol--SequencingResults.png"> | + | <img class="centreimage" width=100% src="https://static.igem.org/mediawiki/2017/5/5d/T--Bristol--SequencingResults.png"> |
<p><br></p> | <p><br></p> | ||
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Revision as of 13:26, 31 October 2017
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PCR results
The following table shows the final conditions used for successful PCR:
An agarose gel of final fragment PCR products is shown below:
Sequencing results
We successfully performed PCR on our fragments with our editing primers, to isolate the whole coding regions. Sequencing of our constructs showed that most had significant deletions and insertions of unknown DNA, however one construct, NrfB pSB1C3, had only one point mutation (shown below). We submitted this part (see our Parts page), annotating the mutation so future teams can take it into consideration when ordering the part. The part can still be used accurately if this mutation is edited, either molecularly, or through DNA synthesis.
