Difference between revisions of "Team:UST Beijing/Experiments"

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<li> Experiments </li>
 
<li> Experiments </li>
 
<li> Documentation of the development of your project </li>
 
<li> Documentation of the development of your project </li>
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<h5>Inspiration</h5>
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  <div class="scroll-link">Parts</div>
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  </a>
 
<ul>
 
<ul>
−
<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Basic_Part">basic_parts</a></li>
−
<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Composite_Part">composite_parts</a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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</ul>
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                        </ul>
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                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Overiew">Overiew</a></li>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Experiments">Experiments</a></li>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Results">Result</a></li>
 +
                  <li><a href="https://2017.igem.org/Team:UST_Beijing/InterLab">InterLab</a></li>
 +
                        </ul>
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 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/Docking Protein">Docking Protein</a></li>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/Simulation">Simulation</a></li>
  
 +
                        </ul></li>
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  <ul>
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              <li><a href="https://2017.igem.org/Team:UST_Beijing/Attributions">Attributions</a></li>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Team">Members</a></li></ul></li>
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<ul>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/HP/Silver">HP/Silver</a></li>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Collaborations">Collaborations</a></li>
 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Modeling/Simmulation">Simmulation</a></li>
  
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 +
                            <li><a href="https://2017.igem.org/Team:UST_Beijing/Sheets">Sheets</a></li></ul></li>
  
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    <h2>Wiki of InterLab by UST_Beijing</h2><br>
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          <h4 class="panel-title"><h3>Background</h3></h4>
 +
        </div>
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        <div id="Background" class="panel-collapse collapse in">
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          <div class="panel-body">
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          <p class="text-justify" align="left">
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          We used a composite part we constructed in 2016 iGEM competition (http://parts.igem.org/Part:BBa_K2072000:Design) for our following experiments. The plasmid pSB1C3/BBa_2072000 was transformed into E.coli BL21(DE3) cells and cultivated in 96-well microplates with following medium components: M9 salt mixture, chloramphenicol, glycerol and specific sugars, e.g. glucose, arabinose, or IPTG. PNPG was also included.  The culture was shaken in 37 degrees C for up to 20 hours. The plate was analyzed with a microplate reader with wavelength at 405 and 620 nm. The data was collected in spreadsheet and analyzed using Matlab.
 +
      </p>
 +
              <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Strain used</h4>
 +
            <p class="text-justify" style="text-align:center" >
  
 +
Escherichia coli BL21(DE3) <br/>
 +
 +
 +
 +
 +
          </p>
 +
<h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Machines</h4>
 +
            <p class="text-justify" style="text-align:center" >
 +
 +
Enspire 2300-001M<br/>
 +
Sigma Laborzentrifugen<br/>
 +
Thermo Scientific Nanodrop 2000<br/>
 +
EVOS AMG microscope<br/>
 +
 +
 +
 +
 +
 +
              <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Methods</h4>
 +
<p class="text-justify" style="text-align:center" >
 +
 +
 +
 +
 +
<h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Protocols</h4>
 +
            <p class="text-justify" style="text-align:center" >
 +
 +
Please click the link:<br/>
 +
<a href="https://2017.igem.org/Competition/InterLab_Study">https://2017.igem.org/Competition/InterLab_Study</a>>
 +
<br/><br/><br/><br/><br/>
 +
 +
 +
          </p>
 +
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 +
          <h4 class="panel-title"><h3>Result</h3></h4>
 +
        </div>
 +
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Revision as of 14:16, 31 October 2017

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UST_Beijing

Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project
    • USTB-Beijing | Welcome

    Wiki of InterLab by UST_Beijing


    We used a composite part we constructed in 2016 iGEM competition (http://parts.igem.org/Part:BBa_K2072000:Design) for our following experiments. The plasmid pSB1C3/BBa_2072000 was transformed into E.coli BL21(DE3) cells and cultivated in 96-well microplates with following medium components: M9 salt mixture, chloramphenicol, glycerol and specific sugars, e.g. glucose, arabinose, or IPTG. PNPG was also included. The culture was shaken in 37 degrees C for up to 20 hours. The plate was analyzed with a microplate reader with wavelength at 405 and 620 nm. The data was collected in spreadsheet and analyzed using Matlab.

    Strain used

    Escherichia coli BL21(DE3)

    Machines

    Enspire 2300-001M
    Sigma Laborzentrifugen
    Thermo Scientific Nanodrop 2000
    EVOS AMG microscope

    Methods

    Protocols

    Please click the link:
    https://2017.igem.org/Competition/InterLab_Study>







    Copyright © 2017 UST_Beijing iGEM. All rights reserved.