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<h4><strong><I>E.Transformation into E. coli DH5α</I></strong> </h4> | <h4><strong><I>E.Transformation into E. coli DH5α</I></strong> </h4> | ||
<p>The ligation product was transformed into E.coli DH5α strain. </p> | <p>The ligation product was transformed into E.coli DH5α strain. </p> | ||
− | <p>If the vector was pET28a, the strain | + | <p>If the vector was pET28a, the strain was grown in LB plate medium containing 10μg/ml kanamycin at 37°C. </p> |
− | <p>If the vector was pBAD30, the strain | + | <p>If the vector was pBAD30, the strain was grown in LB plate medium containing 100μg/ml ampicillin at 37°C. </p> |
− | <p>If the vector was pSB1C3, the | + | <p>If the vector was pSB1C3, the strain was grown in LB plate medium containing 25μg/ml chloramphenicol at 37°C. </p> |
</div> | </div> | ||
</div> | </div> |
Revision as of 14:35, 31 October 2017