Difference between revisions of "Team:INSA-UPS France/Experiments"

Line 33: Line 33:
 
       <p>We engineered here <i>E. coli</i> to mimic for safety reason the presence of <i>Vibrio</i> species.</p>
 
       <p>We engineered here <i>E. coli</i> to mimic for safety reason the presence of <i>Vibrio</i> species.</p>
 
       <ul>
 
       <ul>
−
         <li>Cloning: </li>
+
         <li>Cloning: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
        <li>Integration in chassis: </li>
+
         <li>Validation: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#result1"> successful</a></li>
−
         <li>Validation: </li>
+
 
       </ul>
 
       </ul>
 
     </section>
 
     </section>
Line 43: Line 42:
 
       <p>In this module, we created synthetic communication between our engineered <i>E. coli</i> and <i>V. harveyi</i>.</p>
 
       <p>In this module, we created synthetic communication between our engineered <i>E. coli</i> and <i>V. harveyi</i>.</p>
 
       <ul>
 
       <ul>
−
         <li>Cloning: </li>
+
         <li>Cloning: No cloning for this module</li>
−
         <li>Integration in chassis: </li>
+
         <li>Validation: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
        <li>Validation: </li>
+
 
       </ul>
 
       </ul>
 
     </section>
 
     </section>
Line 53: Line 51:
 
       <p>Here, we present our first effective modification of <i>V. harveyi</i> and its perspectives for the project.</p>
 
       <p>Here, we present our first effective modification of <i>V. harveyi</i> and its perspectives for the project.</p>
 
       <ul>
 
       <ul>
−
         <li>Cloning: </li>
+
         <li>Cloning: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> in progress</a></li>
−
         <li>Integration in chassis: </li>
+
         <li>Integration in chassis: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a> </li>
−
         <li>Validation: </li>
+
         <li>Validation: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
 
       </ul>
 
       </ul>
 
     </section>
 
     </section>
Line 63: Line 61:
 
       <p>This module is dedicated to the production of diacetyl by bacteria, as a signal relay toward yeast.</p>
 
       <p>This module is dedicated to the production of diacetyl by bacteria, as a signal relay toward yeast.</p>
 
       <ul>
 
       <ul>
−
         <li>Cloning: </li>
+
         <li>Cloning: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
        <li>Integration in chassis: </li>
+
         <li>Validation: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
         <li>Validation: </li>
+
 
       </ul>
 
       </ul>
 
     </section>
 
     </section>
Line 73: Line 70:
 
       <p>This presents are modification and first assays to engineered <i>P. pastoris</i> for diacetyl detection.</p>
 
       <p>This presents are modification and first assays to engineered <i>P. pastoris</i> for diacetyl detection.</p>
 
       <ul>
 
       <ul>
−
         <li>Cloning: </li>
+
         <li>Cloning: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
         <li>Integration in chassis: </li>
+
         <li>Integration in chassis: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
         <li>Validation: </li>
+
         <li>Validation: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
 
       </ul>
 
       </ul>
 
     </section>
 
     </section>
Line 83: Line 80:
 
       <p>Finally, this module described the efficiency of a yeast-produced crocodile AMP on <i>V. harveyi</i>.</p>
 
       <p>Finally, this module described the efficiency of a yeast-produced crocodile AMP on <i>V. harveyi</i>.</p>
 
       <ul>
 
       <ul>
−
         <li>Cloning: </li>
+
         <li>Cloning: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
         <li>Integration in chassis: </li>
+
         <li>Integration in chassis: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
−
         <li>Validation: </li>
+
         <li>Validation: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
 
       </ul>
 
       </ul>
 
     </section>
 
     </section>
Line 93: Line 90:
 
       <p>Toward a realistic solution, we tested here how to co-culture how consortium microoganisms.</p>
 
       <p>Toward a realistic solution, we tested here how to co-culture how consortium microoganisms.</p>
 
       <ul>
 
       <ul>
−
         <li>Validation: </li>
+
         <li>Validation: <a href=" https://2017.igem.org/Team:INSA-UPS_France/Protocols#prot7"> successful</a></li>
 
       </ul>
 
       </ul>
 
     </section>
 
     </section>

Revision as of 18:00, 31 October 2017

Croc'n Cholera
iGEM UPS-INSA Toulouse 2017

Experiments Overview

This Realisations section describes all our wet lab work, based on our strategy. We divided this section into the Clonings page and the Results page: the first shows how we integrated the parts of our Design into our chassis, and the second shows the experiments we did to further validate their functionnality. Both are presented for each of our eight modules:

1 - Mimicking Vibrio sp. presence with an engineered E. coli 

We engineered here E. coli to mimic for safety reason the presence of Vibrio species.

2 - E. coli producing C8-CAI-1 molecules can be sensed by V. harveyi 

In this module, we created synthetic communication between our engineered E. coli and V. harveyi.

  • Cloning: No cloning for this module
  • Validation: successful

3 - Modification of V. harveyi to detect both C8-CAI-1 and CAI-1

Here, we present our first effective modification of V. harveyi and its perspectives for the project.

4 - Establishing production of diacetyl to establish communication between prokaryotic and eukaryotic cells

This module is dedicated to the production of diacetyl by bacteria, as a signal relay toward yeast.

5 - Diacetyl detection by Pichia pastoris 

This presents are modification and first assays to engineered P. pastoris for diacetyl detection.

6 - P. pastoris is able to produce functional antimicrobial peptides

Finally, this module described the efficiency of a yeast-produced crocodile AMP on V. harveyi.

7 - Co-cultivate P. pastoris and V. harveyi  is possible

Toward a realistic solution, we tested here how to co-culture how consortium microoganisms.

8 - Membrane permeability assay

We assessed the capacity of our microorganism-impermeable materials to let AMP go through.

  • Validation:
Realisations pages
Overview Notebook Clonings Results Protocols Safety