Difference between revisions of "Team:Harvard/Basic Parts"

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<h1>Parts</h1>
 
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This year we submitted 5 new parts to the parts registry. <br><br>
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The parts feature varying strength RBS in front of csgG, the porous protein involved in the secretion of monomeric csgA in wild-type E. coli. This part can be stitched together with a standard promoter and compared with other similar parts with different RBSs to see some varying level of csgG expression.
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<h2>Origins of Sequences </h2>
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The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the <a href="https://salislab.net/software/"> Salis Lab RBS generator </a>.
  
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<h2>The RBS sequence was generated from a randomized library of RBSs using the <a href="https://salislab.net/software/">Salis Lab RBS generator</a>. Then, plasmids with desired RBS strengths were screened for and selected. Refer to our <a href="https://2017.igem.org/Team:Harvard/Protocols">protocol page</a> for details in procedure. </h2>
  
  
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Revision as of 18:40, 31 October 2017

Harvard OPTI POLY

Parts



This year we submitted 5 new parts to the parts registry.

The parts feature varying strength RBS in front of csgG, the porous protein involved in the secretion of monomeric csgA in wild-type E. coli. This part can be stitched together with a standard promoter and compared with other similar parts with different RBSs to see some varying level of csgG expression.

Origins of Sequences

The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the Salis Lab RBS generator .

The RBS sequence was generated from a randomized library of RBSs using the Salis Lab RBS generator. Then, plasmids with desired RBS strengths were screened for and selected. Refer to our protocol page for details in procedure.