Difference between revisions of "Team:Harvard/Basic Parts"
| Line 4: | Line 4: | ||
<div class="column full_size description" style = "margin-top: 5%"> | <div class="column full_size description" style = "margin-top: 5%"> | ||
<h1>Parts</h1> | <h1>Parts</h1> | ||
| − | + | <center> | |
| − | <img src = "https://static.igem.org/mediawiki/2017/9/94/Harvard--Parts.jpeg"> | + | <img src = "https://static.igem.org/mediawiki/2017/9/94/Harvard--Parts.jpeg"></center> |
<br> | <br> | ||
<br> | <br> | ||
| Line 11: | Line 11: | ||
This year we submitted 5 new parts to the parts registry. <br><br> | This year we submitted 5 new parts to the parts registry. <br><br> | ||
The parts feature varying strength RBS in front of csgG, the porous protein involved in the secretion of monomeric csgA in wild-type E. coli. This part can be stitched together with a standard promoter and compared with other similar parts with different RBSs to see some varying level of csgG expression. | The parts feature varying strength RBS in front of csgG, the porous protein involved in the secretion of monomeric csgA in wild-type E. coli. This part can be stitched together with a standard promoter and compared with other similar parts with different RBSs to see some varying level of csgG expression. | ||
| − | + | <br> | |
| − | + | ||
<h2>Origins of Sequences </h2> | <h2>Origins of Sequences </h2> | ||
The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the <a href="https://salislab.net/software/"> Salis Lab RBS generator </a>. | The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the <a href="https://salislab.net/software/"> Salis Lab RBS generator </a>. | ||
Revision as of 18:41, 31 October 2017
Parts
This year we submitted 5 new parts to the parts registry.
The parts feature varying strength RBS in front of csgG, the porous protein involved in the secretion of monomeric csgA in wild-type E. coli. This part can be stitched together with a standard promoter and compared with other similar parts with different RBSs to see some varying level of csgG expression.
Origins of Sequences
The csgG protein was taken from the wild-type E. coli operon; the specific plasmid was provided by the Joshi lab at the Wyss Institute. The RBS was generated via the Salis Lab RBS generator .Design
The RBS sequence was generated from a randomized library of RBSs using the Salis Lab RBS generator. Then, plasmids with desired RBS strengths were screened for and selected. Refer to our protocol page for details in procedure.
