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Revision as of 19:00, 31 October 2017

HPLC Conditions

Eluent A

2.5 : 97.5 % (v/v) acetic acid: H2O, pH 3.8

*pH was adjusted by addition of 1 mol L-1 KOH

Eluent B

80 : 20 % (v/v) acetonitrile: H2O

Gradient Elution (A>B)

80:20

50:50

0:100

80:20