Difference between revisions of "Team:Newcastle/Results"

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<div class="SOX"><img src="https://static.igem.org/mediawiki/2017/8/89/T--Newcastle--Correct_sox_protein_gel_2.png" width="30%"/>
 
<div class="SOX"><img src="https://static.igem.org/mediawiki/2017/8/89/T--Newcastle--Correct_sox_protein_gel_2.png" width="30%"/>
 
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<p class="legend"><strong>Figure 3:</strong> Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: 6 µl ladder, Lane 2: 10 µl sfGFP, Lane 3: BL21-DE3, Lane 4: 10µl SOX 1, Lane 5: 10 µl SOX 2, Lane 6: 10 µl SOX 3, Lane 7: 10 µl SOX 4, Lane 8: 10 µl SOX 5, Lane 9: 10 µl SOX 6, Lane 10: 6 µl ladder. Circled bands show sarcosine oxidase at ~42 kDa, the expected weight.</p>
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<p class="legend"><center><strong>Figure 3:</strong> Sarcosine Oxidase expression was induced by adding 40 µl of 100 mM IPTG. Lane 1: 6 µl ladder, Lane 2: 10 µl sfGFP, Lane 3: BL21-DE3, Lane 4: 10µl SOX 1, Lane 5: 10 µl SOX 2, Lane 6: 10 µl SOX 3, Lane 7: 10 µl SOX 4, Lane 8: 10 µl SOX 5, Lane 9: 10 µl SOX 6, Lane 10: 6 µl ladder. Circled bands show sarcosine oxidase at ~42 kDa, the expected weight.</p></center>
 
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<div class="SOX"><img src="https://static.igem.org/mediawiki/2017/f/f7/T--Newcastle--Incorrect_sox_gel.jpg" width="30%"/>
 
<div class="SOX"><img src="https://static.igem.org/mediawiki/2017/f/f7/T--Newcastle--Incorrect_sox_gel.jpg" width="30%"/>
 
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<p class="legend"><strong>Figure 4:</strong> Restriction digest confirmation of first sarcosine oxidase assembly using Xba1 and Spe1 restriction enzymes.</p>
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<p class="legend"><center><strong>Figure 4:</strong> Restriction digest confirmation of first sarcosine oxidase assembly using Xba1 and Spe1 restriction enzymes.</p></center>
 
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<div class="SOX"><img src="https://static.igem.org/mediawiki/2017/4/4b/T--Newcastle--SOX_testing.JPG" width="30%"/>
 
<div class="SOX"><img src="https://static.igem.org/mediawiki/2017/4/4b/T--Newcastle--SOX_testing.JPG" width="30%"/>
 
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<p class="legend"><strong>Figure 5:</strong> Lysed cell cultures induced with 40 µl of a 100 mM stock of IPTG tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.</p>
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<p class="legend"><center><strong>Figure 5:</strong> Lysed cell cultures induced with 40 µl of a 100 mM stock of IPTG tested with formaldehyde strips. From left to right: SOX+0 µl sarcosine, SOX+IPTG+0 µl sarcosine, WT+0 µl sarcosine, WT+IPTG+0 µl sarcosine, SOX+50 µl sarcosine, SOX+IPTG+50 µl sarcosine, WT+50 µl sarcosine, WT+IPTG+50 µl sarcosine, SOX+200 µl sarcosine, SOX+IPTG+200 µl, WT+200 µl sarcosine, WT+IPTG+200 µl.</p></center>
 
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<img src="https://static.igem.org/mediawiki/2017/d/db/Vave2.jpg" class="img-fluid border border-dark rounded" style="margin: 2%; max-width: 70%">
 
<img src="https://static.igem.org/mediawiki/2017/d/db/Vave2.jpg" class="img-fluid border border-dark rounded" style="margin: 2%; max-width: 70%">
<div><p class="legend"><strong><b>Figure 2:</b> </strong> The new part <a href="http://parts.igem.org/Part:BBa_K2205022">BBa_K2205022</a> based on the previous Arsenic detector design <a href="http://parts.igem.org/Part:BBa_J33201">BBa_J33201</a>, implemented by the Sensynova part coding for the connector 1, <a href="http://parts.igem.org/Part:BBa_K2205008">BBa_K2205008</a>.</p></div>
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<div><p class="legend"><center><strong><b>Figure 2:</b> </strong> The new part <a href="http://parts.igem.org/Part:BBa_K2205022">BBa_K2205022</a> based on the previous Arsenic detector design <a href="http://parts.igem.org/Part:BBa_J33201">BBa_J33201</a>, implemented by the Sensynova part coding for the connector 1, <a href="http://parts.igem.org/Part:BBa_K2205008">BBa_K2205008</a>.</p></center></div>
  
 
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Revision as of 19:02, 31 October 2017

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Our Experimental Results



Below is a diagram of our Sensynova Framework. Clicking on each part of the framework (e.g. detector modules) links to the relevant results.

Alternatively, at the bottom of this page are tabs which will show you results for every part of the project



Framework

Framework Chassis

Biochemical Adaptor

Target

Detector Modules

Multicellular Framework Testing

C12 HSL: Connector 1

Processor Modules

Framework in Cell Free Protein Synthesis Systems

C4 HSL: Connector 2

Reporter Modules



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