Difference between revisions of "Team:IIT Delhi/recipe"
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| − | + | 20x TAE buffer | |
<ul> | <ul> | ||
<li>80mL of 1M Tris solution | <li>80mL of 1M Tris solution | ||
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| − | <button class="accordion back3" style="font-weight: bold;"> | + | <button class="accordion back3" style="font-weight: bold;">Preparation of alkaline lysis solution I</button> |
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| + | <ul> | ||
| + | <li>50 mM glucose. | ||
| + | <li>25 mM Tris-Cl (pH 8.0). | ||
| + | <li>10 mM EDTA (pH 8.0). | ||
| + | Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by | ||
| + | autoclaving and store at 4°C. | ||
| + | </div> | ||
</div> | </div> | ||
<button class="accordion back4" style="font-weight: bold;">Section 4</button> | <button class="accordion back4" style="font-weight: bold;">Section 4</button> | ||
Revision as of 20:06, 31 October 2017
RECIPES
Accurate Experimenting for Accurate Results !!
- 80mL of 1M Tris solution
- 0.7264g of EDTANa2 salt
- 2.284 mL glacial acetic acid
- distilled H2O 100mL
- 50 mM glucose.
- 25 mM Tris-Cl (pH 8.0).
- 10 mM EDTA (pH 8.0). Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by autoclaving and store at 4°C.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
