Revathireddy (Talk | contribs) |
Revathireddy (Talk | contribs) |
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Results: Agarose gel electrophoresis revealed band of ~7000bp in all colonies tested.<br> | Results: Agarose gel electrophoresis revealed band of ~7000bp in all colonies tested.<br> | ||
To reconfirm, colony 3 (C-3) was inoculated in LB+Amp. Plasmid prep of this colony was performed the next day and confirmed by double digestion with NotI and XhoI. Release of indC fragment (3855bp) was checked by running a 1% agarose gel. We could not see a band of the correct size.<br> | To reconfirm, colony 3 (C-3) was inoculated in LB+Amp. Plasmid prep of this colony was performed the next day and confirmed by double digestion with NotI and XhoI. Release of indC fragment (3855bp) was checked by running a 1% agarose gel. We could not see a band of the correct size.<br> | ||
− | <img src="https://static.igem.org/mediawiki/parts/5/5e/ICT-Mumbai_notebook_gelimage2.png"> | + | <img src="https://static.igem.org/mediawiki/parts/5/5e/ICT-Mumbai_notebook_gelimage2.png"> <br> |
Lane 2 contains 1kb DNA ladder whereas lane 3 contains RE digested pET 43.1a+<i>glnA</i>+<i>indC</i>. | Lane 2 contains 1kb DNA ladder whereas lane 3 contains RE digested pET 43.1a+<i>glnA</i>+<i>indC</i>. | ||
Revision as of 20:20, 31 October 2017