Difference between revisions of "Team:Dalhousie/Results"

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</br><h1><font color= "#C1D35D">Results</font></h1></br>
 
</br><h1><font color= "#C1D35D">Results</font></h1></br>
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<font color= "#C1D35D"><h2>Metagenomic Library</h2></font></br>
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<font color= "#C1D35D">DNA Extraction</font> </br></br>
  
 
Over the course of the 2017 iGEM season, we have had some downs, but many more ups.  
 
Over the course of the 2017 iGEM season, we have had some downs, but many more ups.  
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<font color= "#C1D35D"><h3>Project Achievements<h3></font></br>
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<font color= "#C1D35D"><h3>Project Achievements</h3></font></br>
 
<font color= "#C1D35D">Successes</font></br>
 
<font color= "#C1D35D">Successes</font></br>
  
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</ul>
 
</ul>
  
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<font color= "#C1D35D">Successes</font></br>
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<font color= "#C1D35D">Failures</font></br>
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<ul>
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<li>Did not clone our biobricks into the shipping vector psB1C3</li>
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<li>Was not able to achieve the right environment for our novel beta-xylanase to function</li>
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<li> Was not able to design a functional media assay for our enzymes</li>
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<li> Could not make a functional metagenomic library </li>
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<li> Sheered our high molecular weight DNA</li>
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</ul>
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<font color= "#C1D35D">DNA Extraction</font></br>
 
  
  
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Project Achievements
 
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You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.
 
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A list of linked bullet points of the successful results during your project
 
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A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
 
  
  

Revision as of 20:23, 31 October 2017

Results

Results


Metagenomic Library


DNA Extraction

Over the course of the 2017 iGEM season, we have had some downs, but many more ups.

Project Achievements


Successes
  • Developed a pipeline to identify, or "mine", the porcupine metagenomic sequencing to discover novel enzymes.
  • Identified 8? novel enzymes with variable percent identity.
  • Synthesized 5 of those enzymes, and successfully cloned 4 of them into psB1AK3.
  • Optimized our previous biobrick Endoglucanse(BBa_K2160000)by adding a C termincal HIS-tag and N terminal PelB sequence (Improve).
  • Successfully completed a fluorophore cleavage assay from the Hallam lab.
  • Isolated high molecular weight DNA from porcupine fecal samples.
  • Obtained efficient ligation and digestion with pJC8 controls.
  • Produced phage plaque with the phage packaging extract lamba DNA controls.
Failures
  • Did not clone our biobricks into the shipping vector psB1C3
  • Was not able to achieve the right environment for our novel beta-xylanase to function
  • Was not able to design a functional media assay for our enzymes
  • Could not make a functional metagenomic library
  • Sheered our high molecular weight DNA