Difference between revisions of "Team:IIT Delhi/recipe"
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| − | 0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh) | + | <ul>0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh) |
| − | 1% (w/v) SDS. | + | 1% (w/v) SDS.</ul> |
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<li>H2O, 28.5 ml | <li>H2O, 28.5 ml | ||
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| − | The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. | + | <ul>The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. |
| − | Store the solution at 4°C and transfer it to an ice bucket just before use. | + | Store the solution at 4°C and transfer it to an ice bucket just before use.</ul> |
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Revision as of 20:34, 31 October 2017
RECIPES
Accurate Experimenting for Accurate Results !!
20x TAE buffer
- 80mL of 1M Tris solution
- 0.7264g of EDTANa2 salt
- 2.284 mL glacial acetic acid
- distilled H2O 100mL
- For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap
the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and
store at room temperature.
- 50 mM glucose.
- 25 mM Tris-Cl (pH 8.0).
- 10 mM EDTA (pH 8.0).
- 0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh)
1% (w/v) SDS.
- 5 M potassium acetate, 60.0 ml
- Glacial acetic acid, 11.5 ml
- H2O, 28.5 ml
- The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate.
Store the solution at 4°C and transfer it to an ice bucket just before use.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
