Difference between revisions of "Team:ETH Zurich/Protocols"
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<summary>Bacterial Lysate Production</summary> | <summary>Bacterial Lysate Production</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/ | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/f/fb/T--ETH_Zurich--bacterial_lysate.pdf" width="800"/> |
| − | <a href="https://static.igem.org/mediawiki/2017/ | + | <a href="https://static.igem.org/mediawiki/2017/f/fb/T--ETH_Zurich--bacterial_lysate.pdf" download>Download</a> |
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" width="800"/> | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" width="800"/> | ||
<a href="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" download>Download</a> | <a href="https://static.igem.org/mediawiki/2017/5/59/T--ETH_Zurich--calcein_am_staining_assay.pdf" download>Download</a> | ||
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| + | <summary>Killing Curve of Different Cells Lines with 5-Fluouracil</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" width="800"/> | ||
| + | <a href="https://static.igem.org/mediawiki/2017/2/2a/T--ETH_Zurich--fluouracil_killing_curve.pdf" download>Download</a> | ||
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| − | <summary> | + | <summary>Lysate Tolerance of Different Cell Lines</summary> |
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| − | <a href="https://static.igem.org/mediawiki/2017/ | + | <a href="https://static.igem.org/mediawiki/2017/7/78/T--ETH_Zurich--lysate_tolerance.pdf" download>Download</a> |
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| − | <summary> | + | <summary>Killing Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate</summary> |
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/ | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" width="800"/> |
| − | <a href="https://static.igem.org/mediawiki/2017/ | + | <a href="https://static.igem.org/mediawiki/2017/b/b3/T--ETH_Zurich--azurin_killing_curve.pdf" download>Download</a> |
</details> | </details> | ||
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Revision as of 20:36, 31 October 2017
Protocols
General Protocols
Chemical Transformation
DownloadColony PCR
DownloadConsumables
DownloadCryostock of Bacteria
DownloadElectroporation
DownloadGel Extraction
DownloadGibson Assembly
DownloadLigase Chain Reaction
DownloadMiniprep
DownloadPCR Clean-Up
DownloadPhusion PCR
DownloadPreparation of Chemically Competent Cells
DownloadPreparation of Electrocompetent Cells
DownloadRestriction Ligation
DownloadTumor Sensor
MRI Contrast Agent
Anti-Cancer Toxin
Fluorescence Measurement to Obtain the AHL Dose-Response Curve
DownloadSDS-PAGE to Confirm AHL-Induced Expression of Azurin and p28
DownloadBacterial Lysate Production
DownloadCultivation of mamalian cell lines
DownloadCalcein AM staining assay
DownloadKilling Curve of Different Cells Lines with 5-Fluouracil
DownloadDefining the Seeding Cell Concentration
DownloadLysate Tolerance of Different Cell Lines
DownloadKilling Curve of Different Cell Lines with Azurin-Rich Bacterial Lysate
DownloadHeat Sensor
Heat-Induction of TlpA-controlled GFP Expression
DownloadCell Lysis
Heat-Induction of GFP Release by TlpA-controlled Protein E Expression
Download
