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− | + | <li class="active"><a href="目录.html">Home</a></li> | |
− | + | <li><a href="../portfolio.html">Practice</a></li> | |
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− | <a href=" | + | <a href="../blog.html">Blo</a> |
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− | <li><a href=" | + | <li><a href="#">Design</a></li> |
− | <li><a href=" | + | <li><a href="#">Demonstrate</a></li> |
− | <li><a href=" | + | <li><a href="#">Model</a></li> |
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</ul> | </ul> | ||
</li> | </li> | ||
− | + | <li><a href="../about.html">Achievement</a></li> | |
− | + | <li><a href="../contact.html">Notebook</a></li> | |
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− | <h2> | + | <h2>Journal</h2> |
− | + | <p> </p> | |
+ | <p><span class="label-warning"><font size=4><font color=black> March: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 1-2 (2017.03.04-2017.03.17) | ||
+ | <br> | ||
+ | | ||
+ | After discussion, the main goal of our project was to detect cancer of the liver by using small molecule | ||
+ | <br> | ||
+ | ii. Week 3 (2017.03.18-2017.03.24) | ||
+ | <br> | ||
+ | | ||
+ | After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria. | ||
+ | <br> | ||
+ | |||
+ | iii. week 4 (2017.03.25-2017.03.31)</span><br> | ||
+ | | ||
+ | We chose our main experimental method.<br> | ||
+ | |||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | <p> </p> | ||
+ | <p><span class="label-warning"><font size=4><font color=black> April: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 5 (2017.04.01-2017.04.07) | ||
+ | <br> | ||
+ | | ||
+ | We listed the plasmids we might use, designed the gene lines, began to construct our part. | ||
+ | |||
+ | <br> | ||
+ | ii. Week 6 2017.04.08-2017.04.14) | ||
+ | <br> | ||
+ | | ||
+ | We constructed our first part BBa_K2305008. | ||
+ | <br> | ||
+ | |||
+ | iii. Week 7 (2017.04.15-2017.04.21) | ||
+ | <br> | ||
+ | | ||
+ | We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.<br> | ||
+ | |||
+ | iv. Week 8 (2017.04.22-2017.04.28) | ||
+ | <br> | ||
+ | | ||
+ | We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.<br> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <p> </p> | ||
+ | <p><span class="label-warning"><font size=4><font color=black>May: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 9 (2017.04.29-2017.05.05) | ||
+ | <br> | ||
+ | | ||
+ | We constructed BBa_K2305009、BBa_K2305010. | ||
+ | |||
+ | <br> | ||
+ | ii. Week 10 (2017.05.06-2017.05.12) | ||
+ | <br> | ||
+ | | ||
+ | We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016. | ||
+ | <br> | ||
+ | |||
+ | iii. Week 11 (2017.05.13-2017.05.19) | ||
+ | <br> | ||
+ | | ||
+ | We constructed BBa_K2305007、BBa_K2305006.<br> | ||
+ | |||
+ | iv. Week 12 (2017.05.20-2017.05.26) | ||
+ | <br> | ||
+ | | ||
+ | We constructed BBa_K2305016、BBa_K2305005.<br> | ||
+ | v. Week 13 (2017.05.27-2017.06.02) | ||
+ | <br> | ||
+ | | ||
+ | We constructed BBa_K2305004、BBa_K2305013.<br> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <p> </p> | ||
+ | <p><span class="label-warning"><font size=4><font color=black>June: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 14 (2017.06.03-2017.06.09) | ||
+ | <br> | ||
+ | | ||
+ | We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids. | ||
+ | |||
+ | <br> | ||
+ | ii. Week 15-17 (2017.06.10-2017.06.30) | ||
+ | <br> | ||
+ | | ||
+ | We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids. | ||
+ | <br> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <p> </p> | ||
+ | <p><span class="label-warning"><font size=4><font color=black>July: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 18 (2017.07.01-2017.07.07) | ||
+ | <br> | ||
+ | | ||
+ | We tried to knock out the lysa gene in E.coli by λRed recombination system. | ||
+ | |||
+ | <br> | ||
+ | ii. Week 19 ( 2017.07.08-2017.07.14) | ||
+ | <br> | ||
+ | | ||
+ | We discussed about the problems we met in the gene-knocked. | ||
+ | <br> | ||
+ | |||
+ | iii. Week 20-21 (2017.07.15-2017.07.28) | ||
+ | <br> | ||
+ | | ||
+ | We learned how to produce competent cells and produced enough cells..<br> | ||
+ | </p> | ||
+ | |||
+ | <p> </p> | ||
+ | <p><span class="label-warning"><font size=4><font color=black>August: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 22-23 (2017.07.29-2017.08.16) | ||
+ | <br> | ||
+ | | ||
+ | We import some plasmids we constructed to lysine-deficinent E.coli. | ||
+ | |||
+ | <br> | ||
+ | ii. Week 24-25 ( 2017.08.12-2017.08.25) | ||
+ | <br> | ||
+ | | ||
+ | We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids. | ||
+ | <br> | ||
+ | |||
+ | iii. Week 26 (2017.08.26-2017.09.01) | ||
+ | <br> | ||
+ | | ||
+ | To achieve the standard of silver medal, we sorted out 19 parts completed in totally.<br> | ||
+ | </p> | ||
+ | |||
+ | <p> </p> | ||
+ | <p><span class="label-warning"><font size=4><font color=black> September: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 27-28 (2017.09.02-2017.09.15) | ||
+ | <br> | ||
+ | | ||
+ | To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032) | ||
+ | |||
+ | <br> | ||
+ | ii. Week 29-30 ( 2017.09.16-2017.09.29) | ||
+ | <br> | ||
+ | | ||
+ | To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070 | ||
+ | <br></p> | ||
+ | |||
+ | |||
+ | <p> </p> | ||
+ | <p><span class="label-warning"><font size=4><font color=black>October: </font> </font></span></p> | ||
+ | </span><p> <br> | ||
+ | i. Week 31-32 (2017.09.30-2017.10.13) | ||
+ | <br> | ||
+ | | ||
+ | We sorted out our results and steps of the experiment. | ||
+ | |||
+ | <br> | ||
+ | ii. Week 33-34 (2017.10.14-2017.10.31) | ||
+ | <br> | ||
+ | | ||
+ | We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki. | ||
+ | <br> | ||
+ | |||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
</div> | </div> | ||
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<h2>Hire Us!</h2> | <h2>Hire Us!</h2> | ||
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<p><a href="#" class="btn btn-default btn-lg">Contact Us</a></p> | <p><a href="#" class="btn btn-default btn-lg">Contact Us</a></p> | ||
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<h4>Links</h4> | <h4>Links</h4> | ||
<ul class="fh5co-footer-links"> | <ul class="fh5co-footer-links"> | ||
− | <li><a href=" | + | <li><a href="home.html">Home</a></li> |
<li><a href="#">Team</a></li> | <li><a href="#">Team</a></li> | ||
− | <li><a href=" | + | <li><a href="Achievement.html">Achievement</a></li> |
<li><a href="notebook.html">Notebook</a></li> | <li><a href="notebook.html">Notebook</a></li> | ||
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<div class="gototop js-top"> | <div class="gototop js-top"> |
Revision as of 21:13, 31 October 2017
Journal
March:
i. Week 1-2 (2017.03.04-2017.03.17)
After discussion, the main goal of our project was to detect cancer of the liver by using small molecule
ii. Week 3 (2017.03.18-2017.03.24)
After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria.
iii. week 4 (2017.03.25-2017.03.31)
We chose our main experimental method.
April:
i. Week 5 (2017.04.01-2017.04.07)
We listed the plasmids we might use, designed the gene lines, began to construct our part.
ii. Week 6 2017.04.08-2017.04.14)
We constructed our first part BBa_K2305008.
iii. Week 7 (2017.04.15-2017.04.21)
We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.
iv. Week 8 (2017.04.22-2017.04.28)
We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.
May:
i. Week 9 (2017.04.29-2017.05.05)
We constructed BBa_K2305009、BBa_K2305010.
ii. Week 10 (2017.05.06-2017.05.12)
We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016.
iii. Week 11 (2017.05.13-2017.05.19)
We constructed BBa_K2305007、BBa_K2305006.
iv. Week 12 (2017.05.20-2017.05.26)
We constructed BBa_K2305016、BBa_K2305005.
v. Week 13 (2017.05.27-2017.06.02)
We constructed BBa_K2305004、BBa_K2305013.
June:
i. Week 14 (2017.06.03-2017.06.09)
We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids.
ii. Week 15-17 (2017.06.10-2017.06.30)
We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids.
July:
i. Week 18 (2017.07.01-2017.07.07)
We tried to knock out the lysa gene in E.coli by λRed recombination system.
ii. Week 19 ( 2017.07.08-2017.07.14)
We discussed about the problems we met in the gene-knocked.
iii. Week 20-21 (2017.07.15-2017.07.28)
We learned how to produce competent cells and produced enough cells..
August:
i. Week 22-23 (2017.07.29-2017.08.16)
We import some plasmids we constructed to lysine-deficinent E.coli.
ii. Week 24-25 ( 2017.08.12-2017.08.25)
We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids.
iii. Week 26 (2017.08.26-2017.09.01)
To achieve the standard of silver medal, we sorted out 19 parts completed in totally.
September:
i. Week 27-28 (2017.09.02-2017.09.15)
To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032)
ii. Week 29-30 ( 2017.09.16-2017.09.29)
To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070
October:
i. Week 31-32 (2017.09.30-2017.10.13)
We sorted out our results and steps of the experiment.
ii. Week 33-34 (2017.10.14-2017.10.31)
We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki.
Hire Us!
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