Difference between revisions of "Team:BIT/Model"

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<li class="active"><a href="目录.html">Home</a></li>
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<li><a href="../portfolio.html">Practice</a></li>
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<a>Project</a>
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<li><a href="https://2017.igem.org/Team:BIT/Design">Design</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/Demonstrate">Demonstrate</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/model">Model</a></li>
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<li><a href="https://2017.igem.org/Team:BIT/part">Part</a></li>
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<li><a href="#">Model</a></li>
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<h2>Model</h2>
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<h2>Journal</h2>
−
<p>我猜老外应该看不懂这段话，可以假装这是关于Model的介绍>.<我如果了肥牛去额哦分会如此美味日清晨迷人3恶魔就成人人们v3v吃肉肉吃猫肉i测人发2日3i然后发回去额哦发出你却哦非常年轻饿哦无人过问如果他fqefvjhqefhnewvn1起凤街3i发货发你从i31软件13就03 软件3人3哦夫人r-2
+
    <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black> March: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 1-2 (2017.03.04-2017.03.17)
 +
  <br>
 +
  &nbsp;&nbsp;&nbsp;
 +
After discussion, the main goal of our project was to detect cancer of the liver by using small molecule
  
 +
<br>
 +
ii. Week 3 (2017.03.18-2017.03.24)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria.
 +
<br>
 +
 +
iii. week 4 (2017.03.25-2017.03.31)</span><br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We chose our main experimental method.<br>
 +
 
 +
</p>
 +
 +
 +
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black> April: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 5 (2017.04.01-2017.04.07)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We listed the plasmids we might use, designed the gene lines, began to construct our part.
 +
 +
<br>
 +
ii. Week 6 2017.04.08-2017.04.14)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed our first part BBa_K2305008.
 +
<br>
 +
 +
iii. Week 7 (2017.04.15-2017.04.21)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.<br>
 +
 
 +
iv.    Week 8 (2017.04.22-2017.04.28)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.<br>
 +
</p>
 +
 +
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>May: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 9 (2017.04.29-2017.05.05)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed BBa_K2305009、BBa_K2305010.
 +
 +
<br>
 +
ii. Week 10 (2017.05.06-2017.05.12)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016.
 +
<br>
 +
 +
iii. Week 11 (2017.05.13-2017.05.19)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed BBa_K2305007、BBa_K2305006.<br>
 +
 
 +
iv.    Week 12 (2017.05.20-2017.05.26)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed BBa_K2305016、BBa_K2305005.<br>
 +
v.    Week 13 (2017.05.27-2017.06.02)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We constructed BBa_K2305004、BBa_K2305013.<br>
 +
</p>
 +
 +
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>June: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 14 (2017.06.03-2017.06.09)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids.
 +
 +
<br>
 +
ii. Week 15-17 (2017.06.10-2017.06.30)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids.
 +
<br>
 +
</p>
 +
 +
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>July: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 18 (2017.07.01-2017.07.07)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We tried to knock out the lysa gene in E.coli by λRed recombination system.
 +
 +
<br>
 +
ii. Week 19 ( 2017.07.08-2017.07.14)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We discussed about the problems we met in the gene-knocked.
 +
<br>
 +
 +
iii. Week 20-21 (2017.07.15-2017.07.28)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We learned how to produce competent cells and produced enough cells..<br>
 +
</p>
 +
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>August: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 22-23 (2017.07.29-2017.08.16)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We import some plasmids we constructed to lysine-deficinent E.coli.
 +
 +
<br>
 +
ii. Week 24-25 (  2017.08.12-2017.08.25)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids.
 +
<br>
 +
 +
iii. Week 26 (2017.08.26-2017.09.01)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
To achieve the standard of silver medal, we sorted out 19 parts completed in totally.<br>
 +
</p>
 +
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black> September: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 27-28 (2017.09.02-2017.09.15)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032)
 +
 +
<br>
 +
ii. Week 29-30 (  2017.09.16-2017.09.29)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070
 +
<br></p>
 +
 +
 +
      <p>&nbsp;</p>
 +
<p><span class="label-warning"><font size=4><font color=black>October: </font> </font></span></p>
 +
  </span><p>&nbsp;&nbsp;<br>
 +
i. Week 31-32 (2017.09.30-2017.10.13)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We sorted out our results and steps of the experiment.
 +
 +
<br>
 +
ii. Week 33-34 (2017.10.14-2017.10.31)
 +
<br>
 +
  &nbsp;&nbsp;&nbsp;
 +
We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki.
 +
<br>
 +
 +
</p>
 +
 +
 +
 +
 
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                                            <img src="https://static.igem.org/mediawiki/2017/e/ec/BIT_Figure_About_The_Secondary_Structure_of_AP273.png" alt="Image" class="img-fluid tm-img">
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                                            <figcaption>
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                                                <h2 class="tm-figure-title">Model<span>of</span> <span>Biosensor</span></h2>
+
−
                                           
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−
                                                <a href="https://2017.igem.org/Team:BIT/Model/Biosensor">View more</a>
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                                            </figcaption>         
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                                            <img src="https://static.igem.org/mediawiki/2017/f/f0/BIT_Figure_red_fluorescent.png" alt="Image" class="img-fluid tm-img">
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                                            <figcaption>
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                                                <h2 class="tm-figure-title">Model<span>of</span> <span>Bioamplifier</span></h2>
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                                                <a href="https://2017.igem.org/Team:BIT/Model/Bioamplifer">View more</a>
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                                            </figcaption>         
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<h2>Hire Us!</h2>
 
<h2>Hire Us!</h2>
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<p><a href="#" class="btn btn-default btn-lg">Contact Us</a></p>
 
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<h4>Links</h4>
 
<h4>Links</h4>
 
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<li><a href="https://2017.igem.org/Team:BIT">Home</a></li>
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<li><a href="#">Team</a></li>
 
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<li><a href="https://2017.igem.org/Team:BIT/achievement">Achievement</a></li>
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<li><a href="Achievement.html">Achievement</a></li>
 
<li><a href="notebook.html">Notebook</a></li>
 
<li><a href="notebook.html">Notebook</a></li>
 
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Revision as of 21:13, 31 October 2017

Journal

 

March:

  
i. Week 1-2 (2017.03.04-2017.03.17)
    After discussion, the main goal of our project was to detect cancer of the liver by using small molecule
ii. Week 3 (2017.03.18-2017.03.24)
    After reviewing the literature, we chose lysine as our small molecule and chose lysine-deficient E.coli as our bacteria.
iii. week 4 (2017.03.25-2017.03.31)
    We chose our main experimental method.

 

April:

  
i. Week 5 (2017.04.01-2017.04.07)
    We listed the plasmids we might use, designed the gene lines, began to construct our part.
ii. Week 6 2017.04.08-2017.04.14)
    We constructed our first part BBa_K2305008.
iii. Week 7 (2017.04.15-2017.04.21)
    We constructed our part BBa_K2305011、BBa_K2305012 and BBa_K2305003、BBa_K2305002.
iv. Week 8 (2017.04.22-2017.04.28)
    We import BBa_K876070 to E.coli, detect the growth and fluorescence curve of E.coil under various lysine concentration.

 

May:

  
i. Week 9 (2017.04.29-2017.05.05)
    We constructed BBa_K2305009、BBa_K2305010.
ii. Week 10 (2017.05.06-2017.05.12)
    We constructed BBa_K2305014、BBa_K2305015、BBa_K2305016.
iii. Week 11 (2017.05.13-2017.05.19)
    We constructed BBa_K2305007、BBa_K2305006.
iv. Week 12 (2017.05.20-2017.05.26)
    We constructed BBa_K2305016、BBa_K2305005.
v. Week 13 (2017.05.27-2017.06.02)
    We constructed BBa_K2305004、BBa_K2305013.

 

June:

  
i. Week 14 (2017.06.03-2017.06.09)
    We begin to detect the growth and fluorescence curve of E.coil which imported our plasmids.
ii. Week 15-17 (2017.06.10-2017.06.30)
    We have achieved the detection of the growth and fluoresence curve of E.coli imported our plasmids.

 

July:

  
i. Week 18 (2017.07.01-2017.07.07)
    We tried to knock out the lysa gene in E.coli by λRed recombination system.
ii. Week 19 ( 2017.07.08-2017.07.14)
    We discussed about the problems we met in the gene-knocked.
iii. Week 20-21 (2017.07.15-2017.07.28)
    We learned how to produce competent cells and produced enough cells..

 

August:

  
i. Week 22-23 (2017.07.29-2017.08.16)
    We import some plasmids we constructed to lysine-deficinent E.coli.
ii. Week 24-25 ( 2017.08.12-2017.08.25)
    We detect the growth and fluorescence curve of lysine-deficinent E.coli we had imported our plasmids.
iii. Week 26 (2017.08.26-2017.09.01)
    To achieve the standard of silver medal, we sorted out 19 parts completed in totally.

 

September:

  
i. Week 27-28 (2017.09.02-2017.09.15)
    To reach the gold medal, we worked hard to improve an existing BioBrick, iGEM12_UT_Dallas’s BioBrick(BBa_K876070) and iGEM2006_Imperial’s BioBrick(BBa_J37032)
ii. Week 29-30 ( 2017.09.16-2017.09.29)
    To achieve the standard of the copper medal, we have improved the characterizations of BBa_I13973、BBa_I6088、BBa_K584000、BBa_K876070

 

October:

  
i. Week 31-32 (2017.09.30-2017.10.13)
    We sorted out our results and steps of the experiment.
ii. Week 33-34 (2017.10.14-2017.10.31)
    We freeze-dried our plasmids and mailed it to IGEM, and then finished our wiki.

Hire Us!

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Contact Us