Difference between revisions of "Team:IIT Delhi/recipe"
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| − | + | 20x TAE buffer | |
<ul> | <ul> | ||
<li>80mL of 1M Tris solution | <li>80mL of 1M Tris solution | ||
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<li>distilled H2O 100mL | <li>distilled H2O 100mL | ||
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| − | <ul>For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. | + | <ul> |
| − | Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. | + | <li>For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O. |
| − | the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and | + | <li>Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. |
| + | <li> Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and | ||
store at room temperature. </ul> | store at room temperature. </ul> | ||
| + | <br> | ||
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<ul>Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by | <ul>Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by | ||
autoclaving and store at 4°C.</ul> | autoclaving and store at 4°C.</ul> | ||
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Revision as of 22:32, 31 October 2017
RECIPES
Accurate Experimenting for Accurate Results !!
20x TAE buffer
- 80mL of 1M Tris solution
- 0.7264g of EDTANa2 salt
- 2.284 mL glacial acetic acid
- distilled H2O 100mL
- For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
- Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.
- Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.
- 50 mM glucose.
- 25 mM Tris-Cl (pH 8.0).
- 10 mM EDTA (pH 8.0).
- Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by
autoclaving and store at 4°C.
- 0.2 N NaOH (freshly diluted from a 10 N stock).(100ul SDS + 700ul mq + 200ul NaOH)
1% (w/v) SDS.
- 5 M potassium acetate, 60.0 ml
- Glacial acetic acid, 11.5 ml
- H2O, 28.5 ml
- The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate.
Store the solution at 4°C and transfer it to an ice bucket just before use.
