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| + | <h1>Main Page</h1><br> |
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| | + | <img src="https://static.igem.org/mediawiki/2017/f/f2/T--iTesla-SoundBio--mainpage.jpg" /> |
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| − | | + | <h2><b>About Us</b></h2> |
| − | <div class="column full_size" > | + | <p>Team iTesla-SoundBio is a Washington team started this year by Roya Amini-Naieni and Anna Vasyura. The name comes from Tesla STEM High School and International High School, the schools which most team members come from. However, our team consists of high school students from 7 schools and all grade levels. We work out of SoundBio Lab in Seattle, WA</p><br> |
| − | <img src="http://placehold.it/2000x300/d3d3d3/f2f2f2"> | + | <br> |
| − | </div> | + | <h2>Our Project</h2> |
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| − | <h1> Welcome to iGEM 2017! </h1>
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| − | <p>Your team has been approved and you are ready to start the iGEM season! </p> | + | |
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| − | <h5>Before you start: </h5>
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| − | <p> Please read the following pages:</p>
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| − | <li> <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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| − | <li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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| − | </ul>
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| − | <h5> Styling your wiki </h5>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | </div>
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| − | </div>
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| − | <h5> Wiki template information </h5>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | </div>
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| − | <h5> Editing your wiki </h5>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − | </div>
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| − | <h5>Tips</h5>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | </div>
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| − | <h5>Inspiration</h5>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | <h5> Uploading pictures and files </h5>
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| − | <p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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| − | <a href="https://2017.igem.org/Special:Upload"> | + | |
| − | UPLOAD FILES
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| | </html> | | </html> |
| | + | {{iTesla-SoundBio}} |
Team iTesla-SoundBio is a Washington team started this year by Roya Amini-Naieni and Anna Vasyura. The name comes from Tesla STEM High School and International High School, the schools which most team members come from. However, our team consists of high school students from 7 schools and all grade levels. We work out of SoundBio Lab in Seattle, WA
The production of PCBs has been banned in the United States because of possible impact on animal and human health, yet the harmful molecules still contaminate our waters today. Our project involves transferring genes responsible for producing PCB-dechlorinating enzymes from the anaerobic D. mccartyi bacterium, in which they're naturally found, into E.coli, an easier-to-work-with bacterium.
We will be transferring three different genetic variants of the PCB dechlorinating enzyme and will face many challenges in producing the working enzymes. The enzymes probably use norpseudo-B12 as a cofactor, a compound E. coli doesn't naturally create. We are currently researching ways to introduce this into the environment of the E.coli expressing the proteins. The working enzymes also contain iron-sulfer clusters, which will be destroyed upon exposure to oxygen, as the original organism is anaerobic. We are currently looking into different and easy ways to grow E.coli anaerobically and ways to combat this issue. D. mccartyi's membrane is also archea-like and dissimilar to E.coli's membrane, which may cause complications with expression and localization. The enzymes are also transmembrane proteins, which may pose problems.
We will be contacting other labs with higher biosafety levels due to the toxicity of PCBs, to test out our system. We are currently working in lab to transfer our synthesized genetic circuits to E.coli bacteria while we research ways to solve the potential problems listed above. We have also included His tags in our genetic circuits and have partnered with the Baker Lab at the University of Washington to analyze our PCB dechlorinating enzymes in the future.
