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<p><h2>Collaboration with HKUST</h2></p> | <p><h2>Collaboration with HKUST</h2></p> | ||
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> | ||
− | The Hong Kong HKUST team aspire to create a safety switch that would not compromise the functionality of original biobrick, but able to revert the transformant back to wild-type with time. We helped HKUST team’s progress by providing them a Cython-implemented Python interface. It enables them to use the ViennaRNA package functions(written in C language) in Python interface, so they can easily model the dissociation constant for the antisense mRNA and transcript mRNA. | + | The <a href="https://2017.igem.org/Team:Hong_Kong_HKUST/Collaborations"> Hong Kong HKUST team</a> aspire to create a safety switch that would not compromise the functionality of original biobrick, but able to revert the transformant back to wild-type with time. We helped HKUST team’s progress by providing them a Cython-implemented Python interface. It enables them to use the ViennaRNA package functions(written in C language) in Python interface, so they can easily model the dissociation constant for the antisense mRNA and transcript mRNA. |
We also help characterizing their biobricks by running an GFP assay, detailed protocol and results attached below. In return, they provided us two Python scripts for our modelling of free energies of opened and closed toehold switches, by plotting ODE-based model and stochastic model using Euler’s method and Gillespie algorithm respectively. This facilitates our screening of optimal toehold switches in our program.</p> | We also help characterizing their biobricks by running an GFP assay, detailed protocol and results attached below. In return, they provided us two Python scripts for our modelling of free energies of opened and closed toehold switches, by plotting ODE-based model and stochastic model using Euler’s method and Gillespie algorithm respectively. This facilitates our screening of optimal toehold switches in our program.</p> | ||
<center> <img src="https://static.igem.org/mediawiki/2017/5/5d/CUHK_UST.jpg" width="450px" height="auto"> </center> | <center> <img src="https://static.igem.org/mediawiki/2017/5/5d/CUHK_UST.jpg" width="450px" height="auto"> </center> |
Revision as of 08:05, 1 November 2017