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In the first figure you can see that we used excess of the Inducers, as there is a decrease of the amount, but after a while stays at the same amount. This is because all the pockets of the scaffold construct are already filled with the inducer. Data supporting the inducer excess is also shown in the figure, as the amount of empty scaffold sites decreases as fast as the inducer. Furthermore, we see that the induced scaffold sites are very high in the beginning, actually, the induced amount of scaffold sites starts at 0, but increases so fast, that it isn’t visible in a logarithmic time axis. Another thing that stands out in the graph, is that the green graph (the one of the Binding Sites for the Scaffold) isn’t visible in the figure, this is because these values are about the same as the ones of the induced scaffold sites. They decrease with the same rate, because they both are used for the formation of a bond to create a complex. </br></br> | In the first figure you can see that we used excess of the Inducers, as there is a decrease of the amount, but after a while stays at the same amount. This is because all the pockets of the scaffold construct are already filled with the inducer. Data supporting the inducer excess is also shown in the figure, as the amount of empty scaffold sites decreases as fast as the inducer. Furthermore, we see that the induced scaffold sites are very high in the beginning, actually, the induced amount of scaffold sites starts at 0, but increases so fast, that it isn’t visible in a logarithmic time axis. Another thing that stands out in the graph, is that the green graph (the one of the Binding Sites for the Scaffold) isn’t visible in the figure, this is because these values are about the same as the ones of the induced scaffold sites. They decrease with the same rate, because they both are used for the formation of a bond to create a complex. </br></br> | ||
− | <div | + | <div class="Figure_1"><img src="https://static.igem.org/mediawiki/2017/c/c8/T--TU-Eindhoven--Model_result_fig2.png" width="500" height="375" alt="Figure_2_of_model_part" /> |
<figcaption>Figure 1: Graph of the amount of molecules (sites) over time</figcaption></div> </br> | <figcaption>Figure 1: Graph of the amount of molecules (sites) over time</figcaption></div> </br> | ||
In the second graph, we can see how many complexes are formed. With the programs provided by NFsim, we could extract the total amount of molecules and the average complex size. They also provided us with a method to determine the amount of single molecules (they call it complexes with size one) and we used that to determine the amount of formed complexes and the average complex size, where the single molecules aren’t taken into account. Just as we mentioned as comment for the first figure, also here the initial values of Complexes and Average Complex size >1 start at zero, but isn’t visible in the figure because of the logarithmic scale. </br></br> | In the second graph, we can see how many complexes are formed. With the programs provided by NFsim, we could extract the total amount of molecules and the average complex size. They also provided us with a method to determine the amount of single molecules (they call it complexes with size one) and we used that to determine the amount of formed complexes and the average complex size, where the single molecules aren’t taken into account. Just as we mentioned as comment for the first figure, also here the initial values of Complexes and Average Complex size >1 start at zero, but isn’t visible in the figure because of the logarithmic scale. </br></br> | ||
− | <div | + | <div class="Figure_2"><img src="https://static.igem.org/mediawiki/2017/c/c8/T--TU-Eindhoven--Model_result_fig2.png" width="500" height="375" alt="Figure_2_of_model_part" /> |
<figcaption>Figure 2: Graph of the amount of molecules and complexes over time</figcaption> | <figcaption>Figure 2: Graph of the amount of molecules and complexes over time</figcaption> | ||
</div> </br> | </div> </br> |
Revision as of 08:47, 1 November 2017