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| − | <html xmlns:v="urn:schemas-microsoft-com:vml"
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| − | xmlns:o="urn:schemas-microsoft-com:office:office"
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| − | xmlns:w="urn:schemas-microsoft-com:office:word"
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| − | xmlns:m="http://schemas.microsoft.com/office/2004/12/omml"
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| − | xmlns="http://www.w3.org/TR/REC-html40">
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| | | | |
| − | <head>
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| − | <meta http-equiv=Content-Type content="text/html; charset=windows-1252">
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| − | <meta name=ProgId content=Word.Document>
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| − | <meta name=Generator content="Microsoft Word 12">
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| − | <meta name=Originator content="Microsoft Word 12">
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| − | <link rel=File-List href="Protocol_files/filelist.xml">
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| − | <link rel=Edit-Time-Data href="Protocol_files/editdata.mso">
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| − | <!--[if !mso]>
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| − | <style>
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| − | v\:* {behavior:url(#default#VML);}
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| − | o\:* {behavior:url(#default#VML);}
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| − | w\:* {behavior:url(#default#VML);}
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| − | .shape {behavior:url(#default#VML);}
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| − | </style>
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| − | <![endif]--><!--[if gte mso 9]><xml>
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| − | <o:DocumentProperties>
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| − | <o:Author>hp</o:Author>
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| − | <o:LastAuthor>shivi</o:LastAuthor>
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| − | <o:Revision>2</o:Revision>
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| − | <o:TotalTime>2</o:TotalTime>
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| − | <o:Created>2017-11-01T07:50:00Z</o:Created>
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| − | <o:LastSaved>2017-11-01T07:50:00Z</o:LastSaved>
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| − | <o:Pages>3</o:Pages>
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| − | <o:Words>14</o:Words>
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| − | <o:Characters>84</o:Characters>
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| − | <o:Company>HP</o:Company>
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| − | <o:Lines>1</o:Lines>
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| − | <o:Paragraphs>1</o:Paragraphs>
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| − | <o:CharactersWithSpaces>97</o:CharactersWithSpaces>
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| − | <o:Version>12.00</o:Version>
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| − | </o:DocumentProperties>
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| − | <o:OfficeDocumentSettings>
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| − | <o:AllowPNG/>
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| − | </o:OfficeDocumentSettings>
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| − | </xml><![endif]-->
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| − | <link rel=dataStoreItem href="Protocol_files/item0001.xml"
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| − | target="Protocol_files/props0002.xml">
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| − | <link rel=themeData href="Protocol_files/themedata.thmx">
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| − | <link rel=colorSchemeMapping href="Protocol_files/colorschememapping.xml">
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| − | <!--[if gte mso 9]><xml>
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| − | <w:WordDocument>
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| − | <w:HideSpellingErrors/>
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| − | <w:HideGrammaticalErrors/>
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| − | <w:TrackFormatting/>
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| − | <w:PunctuationKerning/>
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| − | <w:ValidateAgainstSchemas/>
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| − | <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid>
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| − | <w:IgnoreMixedContent>false</w:IgnoreMixedContent>
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| − | <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText>
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| − | <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript>
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| − | <w:Compatibility>
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| − | <w:BreakWrappedTables/>
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| − | <w:SnapToGridInCell/>
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| − | <w:WrapTextWithPunct/>
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| − | <w:UseAsianBreakRules/>
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| − | <w:DontGrowAutofit/>
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| − | <w:SplitPgBreakAndParaMark/>
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| − | <w:DontVertAlignCellWithSp/>
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| − | <w:DontBreakConstrainedForcedTables/>
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| − | <w:DontVertAlignInTxbx/>
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| − | <w:Word11KerningPairs/>
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| − | <w:CachedColBalance/>
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| − | </w:Compatibility>
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| − | <m:smallFrac/>
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| − | <m:dispDef/>
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| − | <m:rMargin m:val="0"/>
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| − | <m:defJc m:val="centerGroup"/>
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| − | <m:wrapIndent m:val="1440"/>
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| − | <m:intLim m:val="subSup"/>
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| − | <m:naryLim m:val="undOvr"/>
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| − | </m:mathPr></w:WordDocument>
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| − | </xml><![endif]--><!--[if gte mso 9]><xml>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 2"/>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 3"/>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 4"/>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 5"/>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 6"/>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 7"/>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 8"/>
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| − | <w:LsdException Locked="false" Priority="9" QFormat="true" Name="heading 9"/>
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| − | <w:LsdException Locked="false" Priority="39" Name="toc 2"/>
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| − | <w:LsdException Locked="false" Priority="39" Name="toc 4"/>
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| − | <w:LsdException Locked="false" Priority="39" Name="toc 5"/>
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| − | <w:LsdException Locked="false" Priority="39" Name="toc 6"/>
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| − | <w:LsdException Locked="false" Priority="39" Name="toc 7"/>
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| − | <w:LsdException Locked="false" Priority="39" Name="toc 8"/>
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| − | <w:LsdException Locked="false" Priority="39" Name="toc 9"/>
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| − | <w:LsdException Locked="false" UnhideWhenUsed="false" Name="Placeholder Text"/>
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| − | UnhideWhenUsed="false" QFormat="true" Name="No Spacing"/>
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| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light List"/>
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| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Grid"/>
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| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false"
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| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 2"/>
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| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 1"/>
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| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 1"/>
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| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 2"/>
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| − | UnhideWhenUsed="false" Name="Medium Grid 3"/>
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| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false"
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| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false"
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| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful List"/>
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| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful Grid"/>
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| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Shading Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light List Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Grid Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 1 Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 2 Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 1 Accent 1"/>
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| − | <w:LsdException Locked="false" UnhideWhenUsed="false" Name="Revision"/>
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| − | <w:LsdException Locked="false" Priority="34" SemiHidden="false"
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| − | UnhideWhenUsed="false" QFormat="true" Name="List Paragraph"/>
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| − | <w:LsdException Locked="false" Priority="29" SemiHidden="false"
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| − | UnhideWhenUsed="false" QFormat="true" Name="Quote"/>
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| − | <w:LsdException Locked="false" Priority="30" SemiHidden="false"
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| − | UnhideWhenUsed="false" QFormat="true" Name="Intense Quote"/>
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| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 2 Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 1 Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 2 Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 3 Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Dark List Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful Shading Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful List Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful Grid Accent 1"/>
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| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Shading Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light List Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Grid Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 1 Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 2 Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 1 Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 2 Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 1 Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 2 Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 3 Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Dark List Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful Shading Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful List Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful Grid Accent 2"/>
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| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Shading Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light List Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Grid Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 1 Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 2 Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 1 Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 2 Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 1 Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 2 Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 3 Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Dark List Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful Shading Accent 3"/>
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| − | UnhideWhenUsed="false" Name="Colorful List Accent 3"/>
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| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Colorful Grid Accent 3"/>
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| − | UnhideWhenUsed="false" Name="Light Shading Accent 4"/>
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| − | UnhideWhenUsed="false" Name="Light List Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Grid Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 1 Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Shading 2 Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium List 1 Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 2 Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 1 Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Grid 2 Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Grid 3 Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Dark List Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful Shading Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful List Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful Grid Accent 4"/>
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| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Light Shading Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Light List Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Light Grid Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Shading 1 Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Shading 2 Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium List 1 Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium List 2 Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 1 Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Grid 2 Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Grid 3 Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Dark List Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful Shading Accent 5"/>
| |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful List Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful Grid Accent 5"/>
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| − | <w:LsdException Locked="false" Priority="60" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Light Shading Accent 6"/>
| |
| − | <w:LsdException Locked="false" Priority="61" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Light List Accent 6"/>
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| − | <w:LsdException Locked="false" Priority="62" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Light Grid Accent 6"/>
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| − | <w:LsdException Locked="false" Priority="63" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Shading 1 Accent 6"/>
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| − | <w:LsdException Locked="false" Priority="64" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Shading 2 Accent 6"/>
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| − | <w:LsdException Locked="false" Priority="65" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium List 1 Accent 6"/>
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| − | <w:LsdException Locked="false" Priority="66" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium List 2 Accent 6"/>
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| − | <w:LsdException Locked="false" Priority="67" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Grid 1 Accent 6"/>
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| − | <w:LsdException Locked="false" Priority="68" SemiHidden="false"
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| − | UnhideWhenUsed="false" Name="Medium Grid 2 Accent 6"/>
| |
| − | <w:LsdException Locked="false" Priority="69" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Medium Grid 3 Accent 6"/>
| |
| − | <w:LsdException Locked="false" Priority="70" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Dark List Accent 6"/>
| |
| − | <w:LsdException Locked="false" Priority="71" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful Shading Accent 6"/>
| |
| − | <w:LsdException Locked="false" Priority="72" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful List Accent 6"/>
| |
| − | <w:LsdException Locked="false" Priority="73" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" Name="Colorful Grid Accent 6"/>
| |
| − | <w:LsdException Locked="false" Priority="19" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" QFormat="true" Name="Subtle Emphasis"/>
| |
| − | <w:LsdException Locked="false" Priority="21" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" QFormat="true" Name="Intense Emphasis"/>
| |
| − | <w:LsdException Locked="false" Priority="31" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" QFormat="true" Name="Subtle Reference"/>
| |
| − | <w:LsdException Locked="false" Priority="32" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" QFormat="true" Name="Intense Reference"/>
| |
| − | <w:LsdException Locked="false" Priority="33" SemiHidden="false"
| |
| − | UnhideWhenUsed="false" QFormat="true" Name="Book Title"/>
| |
| − | <w:LsdException Locked="false" Priority="37" Name="Bibliography"/>
| |
| − | <w:LsdException Locked="false" Priority="39" QFormat="true" Name="TOC Heading"/>
| |
| − | </w:LatentStyles>
| |
| − | </xml><![endif]-->
| |
| − | <style>
| |
| − | <!--
| |
| − | /* Font Definitions */
| |
| − | @font-face
| |
| − | {font-family:"Cambria Math";
| |
| − | panose-1:2 4 5 3 5 4 6 3 2 4;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:roman;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:-536870145 1107305727 0 0 415 0;}
| |
| − | @font-face
| |
| − | {font-family:Calibri;
| |
| − | panose-1:2 15 5 2 2 2 4 3 2 4;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:swiss;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:-536859905 -1073732485 9 0 511 0;}
| |
| − | @font-face
| |
| − | {font-family:Tahoma;
| |
| − | panose-1:2 11 6 4 3 5 4 4 2 4;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:swiss;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:-520081665 -1073717157 41 0 66047 0;}
| |
| − | @font-face
| |
| − | {font-family:Algerian;
| |
| − | panose-1:4 2 7 5 4 10 2 6 7 2;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:decorative;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:3 0 0 0 1 0;}
| |
| − | @font-face
| |
| − | {font-family:"Book Antiqua";
| |
| − | panose-1:2 4 6 2 5 3 5 3 3 4;
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:roman;
| |
| − | mso-font-pitch:variable;
| |
| − | mso-font-signature:647 0 0 0 159 0;}
| |
| − | @font-face
| |
| − | {font-family:"Open Sans";
| |
| − | panose-1:0 0 0 0 0 0 0 0 0 0;
| |
| − | mso-font-alt:"Times New Roman";
| |
| − | mso-font-charset:0;
| |
| − | mso-generic-font-family:roman;
| |
| − | mso-font-format:other;
| |
| − | mso-font-pitch:auto;
| |
| − | mso-font-signature:0 0 0 0 0 0;}
| |
| − | /* Style Definitions */
| |
| − | p.MsoNormal, li.MsoNormal, div.MsoNormal
| |
| − | {mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-parent:"";
| |
| − | margin-top:0cm;
| |
| − | margin-right:0cm;
| |
| − | margin-bottom:10.0pt;
| |
| − | margin-left:0cm;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | a:link, span.MsoHyperlink
| |
| − | {mso-style-priority:99;
| |
| − | color:blue;
| |
| − | text-decoration:underline;
| |
| − | text-underline:single;}
| |
| − | a:visited, span.MsoHyperlinkFollowed
| |
| − | {mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | color:purple;
| |
| − | mso-themecolor:followedhyperlink;
| |
| − | text-decoration:underline;
| |
| − | text-underline:single;}
| |
| − | p
| |
| − | {mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | mso-margin-top-alt:auto;
| |
| − | margin-right:0cm;
| |
| − | mso-margin-bottom-alt:auto;
| |
| − | margin-left:0cm;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:12.0pt;
| |
| − | font-family:"Times New Roman","serif";
| |
| − | mso-fareast-font-family:"Times New Roman";
| |
| − | mso-fareast-theme-font:minor-fareast;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
| |
| − | {mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | mso-style-link:"Balloon Text Char";
| |
| − | margin:0cm;
| |
| − | margin-bottom:.0001pt;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:8.0pt;
| |
| − | font-family:"Tahoma","sans-serif";
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | margin-top:0cm;
| |
| − | margin-right:0cm;
| |
| − | margin-bottom:10.0pt;
| |
| − | margin-left:36.0pt;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | p.MsoListParagraphCxSpFirst, li.MsoListParagraphCxSpFirst, div.MsoListParagraphCxSpFirst
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-type:export-only;
| |
| − | margin-top:0cm;
| |
| − | margin-right:0cm;
| |
| − | margin-bottom:0cm;
| |
| − | margin-left:36.0pt;
| |
| − | margin-bottom:.0001pt;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | p.MsoListParagraphCxSpMiddle, li.MsoListParagraphCxSpMiddle, div.MsoListParagraphCxSpMiddle
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-type:export-only;
| |
| − | margin-top:0cm;
| |
| − | margin-right:0cm;
| |
| − | margin-bottom:0cm;
| |
| − | margin-left:36.0pt;
| |
| − | margin-bottom:.0001pt;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | p.MsoListParagraphCxSpLast, li.MsoListParagraphCxSpLast, div.MsoListParagraphCxSpLast
| |
| − | {mso-style-priority:34;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-type:export-only;
| |
| − | margin-top:0cm;
| |
| − | margin-right:0cm;
| |
| − | margin-bottom:10.0pt;
| |
| − | margin-left:36.0pt;
| |
| − | mso-add-space:auto;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | span.BalloonTextChar
| |
| − | {mso-style-name:"Balloon Text Char";
| |
| − | mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | mso-style-unhide:no;
| |
| − | mso-style-locked:yes;
| |
| − | mso-style-link:"Balloon Text";
| |
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| |
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| |
| − | font-family:"Tahoma","sans-serif";
| |
| − | mso-ascii-font-family:Tahoma;
| |
| − | mso-hansi-font-family:Tahoma;
| |
| − | mso-bidi-font-family:Tahoma;}
| |
| − | .MsoChpDefault
| |
| − | {mso-style-type:export-only;
| |
| − | mso-default-props:yes;
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-fareast-font-family:Calibri;
| |
| − | mso-fareast-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | .MsoPapDefault
| |
| − | {mso-style-type:export-only;
| |
| − | margin-bottom:10.0pt;
| |
| − | line-height:115%;}
| |
| − | @page Section1
| |
| − | {size:612.0pt 792.0pt;
| |
| − | margin:72.0pt 72.0pt 72.0pt 72.0pt;
| |
| − | mso-header-margin:36.0pt;
| |
| − | mso-footer-margin:36.0pt;
| |
| − | mso-paper-source:0;}
| |
| − | div.Section1
| |
| − | {page:Section1;}
| |
| − | /* List Definitions */
| |
| − | @list l0
| |
| − | {mso-list-id:869534640;
| |
| − | mso-list-type:hybrid;
| |
| − | mso-list-template-ids:366508600 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
| |
| − | @list l0:level1
| |
| − | {mso-level-tab-stop:none;
| |
| − | mso-level-number-position:left;
| |
| − | text-indent:-18.0pt;}
| |
| − | ol
| |
| − | {margin-bottom:0cm;}
| |
| − | ul
| |
| − | {margin-bottom:0cm;}
| |
| − | -->
| |
| − | </style>
| |
| − | <!--[if gte mso 10]>
| |
| − | <style>
| |
| − | /* Style Definitions */
| |
| − | table.MsoNormalTable
| |
| − | {mso-style-name:"Table Normal";
| |
| − | mso-tstyle-rowband-size:0;
| |
| − | mso-tstyle-colband-size:0;
| |
| − | mso-style-noshow:yes;
| |
| − | mso-style-priority:99;
| |
| − | mso-style-qformat:yes;
| |
| − | mso-style-parent:"";
| |
| − | mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
| |
| − | mso-para-margin-top:0cm;
| |
| − | mso-para-margin-right:0cm;
| |
| − | mso-para-margin-bottom:10.0pt;
| |
| − | mso-para-margin-left:0cm;
| |
| − | line-height:115%;
| |
| − | mso-pagination:widow-orphan;
| |
| − | font-size:11.0pt;
| |
| − | font-family:"Calibri","sans-serif";
| |
| − | mso-ascii-font-family:Calibri;
| |
| − | mso-ascii-theme-font:minor-latin;
| |
| − | mso-hansi-font-family:Calibri;
| |
| − | mso-hansi-theme-font:minor-latin;
| |
| − | mso-bidi-font-family:"Times New Roman";
| |
| − | mso-bidi-theme-font:minor-bidi;
| |
| − | mso-ansi-language:EN-US;
| |
| − | mso-fareast-language:EN-US;}
| |
| − | </style>
| |
| − | <![endif]--><!--[if gte mso 9]><xml>
| |
| − | <o:shapedefaults v:ext="edit" spidmax="2050"/>
| |
| − | </xml><![endif]--><!--[if gte mso 9]><xml>
| |
| − | <o:shapelayout v:ext="edit">
| |
| − | <o:idmap v:ext="edit" data="1"/>
| |
| − | </o:shapelayout></xml><![endif]-->
| |
| − | </head>
| |
| − |
| |
| − | <body lang=EN-IN link=blue vlink=purple style='tab-interval:36.0pt'>
| |
| − |
| |
| − | <div class=Section1>
| |
| − |
| |
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| − | height:278pt;z-index:6;visibility:visible;mso-width-relative:margin;
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| − | 12.0pt'>Promoter Characterization</span></b><span lang=EN-US
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| − | 12.0pt'>iGEM student had taken part in <i>In-Silico</i> Characterization of
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| − | mso-themetint:242;mso-font-kerning:12.0pt'>Quantity Estimation of Protein:
| |
| − | For quantify the expressed protein Bradford Method is used as given on the
| |
| − | following link </span><span lang=EN-US style='font-size:14.0pt;color:#0D0D0D;
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| − | mso-themetint:242;mso-font-kerning:12.0pt'>Quality Estimation of
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| − | protein:<span style='mso-spacerun:yes'> </span>The quality of expressed
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| − | protein was done by SDS-PAGE.</span><span lang=EN-US style='font-size:
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| − | mso-themetint:242;mso-font-kerning:12.0pt'>Protocol for the SD</span><span
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| − | <p class=MsoNormal><span lang=EN-US>References::</span></p>
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| − | <p class=MsoListParagraphCxSpFirst style='text-indent:-18.0pt;mso-list:
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| − | l0 level1 lfo1'><![if !supportLists]><span lang=EN-US style='mso-bidi-font-family:
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| − | Calibri;mso-bidi-theme-font:minor-latin'><span style='mso-list:Ignore'>1.<span
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| − | style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span
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| − | lang=EN-US>Dhall P, Kumar R and Kumar A. 2012. BOD beads – a ready to use
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| − | seeding material for estimation of organic load of wastewater. Anal.
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| − | Methods, 2012, 4, 4101. DOI: 10.1039/c2ay25960e</span></p>
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| − | <p class=MsoListParagraphCxSpMiddle style='text-indent:-18.0pt;mso-list:
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| − | l0 level1 lfo1'><![if !supportLists]><span lang=EN-US style='mso-bidi-font-family:
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| − | style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span
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| − | lang=EN-US style='font-size:10.5pt;line-height:115%;font-family:"Open Sans","serif";
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| − | color:black;background:white'>He, F. (2011). Bradford Protein Assay. <em><span
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| − | style='font-family:"Open Sans","serif";mso-bidi-font-family:"Times New Roman";
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| − | mso-bidi-theme-font:minor-bidi'>Bio-protocol</span></em> Bio101: e45.
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| − | DOI: </span><span lang=EN-US><a
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| − | href="https://doi.org/10.21769/BioProtoc.45"><span style='font-size:10.5pt;
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| − | line-height:115%;font-family:"Open Sans","serif";color:#0094FF;background:
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| − | white'>10.21769/BioProtoc.45</span></a></span><span lang=EN-US
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| − | style='font-size:10.5pt;line-height:115%;font-family:"Open Sans","serif";
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| − | color:black;background:white'>.</span></p>
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| − | alt="Text Box: References:: 1. Dhall P, Kumar R and Kumar A. 2012. BOD beads – a ready to use seeding material for estimation of organic load of wastewater. Anal. Methods, 2012, 4, 4101. DOI: 10.1039/c2ay25960e 2. He, F. (2011). Bradford Protein Assay. Bio-protocol Bio101: e45. DOI: 10.21769/BioProtoc.45. "
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