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| − | Best basic part content | + | Best basic part content |
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| − | We present a codon-optimized version of the cytochrome c protein that is able to convert silicon educts to organosilicon products.
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| − | This cytochrome c variant provides an easy to use tool that is accessible to everyone in the synthetic biology community and allows the user to harness the vast potential of organosilicons.
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| − | This basic part exhibits a strong tendency to form silicon-carbon bonds and is, therefore, a valuable addition to perform controlled organic chemistry in microorganisms.
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| − | A triple mutant of this part has already been applied in the successful synthesis of organosilicons as a proof-of-concept.
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| − | As a next step, this part can be implemented in the directed evolution approach of phage-assisted continuous evolution (PACE) or in the phage-related discontinuous evolution (PREDCEL) approach to improve organosilicon synthesis by cytochrome engineering.
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