Difference between revisions of "Team:IIT Delhi/recipe"

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background-position: center;
 
background-position: center;
 
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color: #ffffff;
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.dropbtn2 {
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    font-size: 16px;   
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    border: none;
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    outline: none;
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    color: white;
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    padding: 15px 16px;
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    background-color: #000;
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    background-size: 100% 100%;
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.navbar a:hover, .dropdown:hover .dropbtn {
 
.navbar a:hover, .dropdown:hover .dropbtn {
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<div class = "navbar ">
 
<div class = "navbar ">
 
 
                                                         <button class="dropbtn1"><a href="https://2017.igem.org/Team:IIT_Delhi">iGEM IIT Delhi</a></button>
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                                                         <button class="dropbtn2"><a href="https://2017.igem.org/Team:IIT_Delhi">iGEM IIT Delhi</a></button>
<div class = "right_menu">
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        <div class = "right_menu">
 
   <div class="dropdown">
 
   <div class="dropdown">
 
     <button class="dropbtn">Project  
 
     <button class="dropbtn">Project  
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     </button>
 
     </button>
 
     <div class="dropdown-content">
 
     <div class="dropdown-content">
       <a href="/Team:IIT_Delhi/Description">Description</a>
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       <a href="/Team:IIT_Delhi/Description">Overview</a>
       <a href="/Team:IIT_Delhi/Results">Results</a>
+
       <a href="/Team:IIT_Delhi/Design">Squarewave Generator</a>
  
       <a href="/Team:IIT_Delhi/Interlab">Interlab</a>
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       <a href="/Team:IIT_Delhi/InterLab">Interlab</a>
 
     </div>
 
     </div>
 
   </div>  
 
   </div>  
 
                                                         <div class="dropdown">
 
                                                         <div class="dropdown">
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    <button class="dropbtn">Results
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    <i class="fa fa-caret-down"></i>
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    </button>
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    <div class="dropdown-content">
 +
      <a href="/Team:IIT_Delhi/Circuit_Design">Circuit design and construction</a>
 +
      <a href="/Team:IIT_Delhi/Microfluidics">Microfluidics and Fluroscence</a>
 +
      <a href="/Team:IIT_Delhi/Photobleaching">Photobleaching</a>
 +
      <a href="/Team:IIT_Delhi/Promoter">Promoter strength</a>
 +
                                                      <a href="/Team:IIT_Delhi/Oscillations">Oscillations</a>
 +
                                                     
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    </div>
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  </div>
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                                                          <div class="dropdown">
 
     <button class="dropbtn">Parts  
 
     <button class="dropbtn">Parts  
 
     <i class="fa fa-caret-down"></i>
 
     <i class="fa fa-caret-down"></i>
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       <a href="/Team:IIT_Delhi/Improved_Part">Improved Parts</a>
 
       <a href="/Team:IIT_Delhi/Improved_Part">Improved Parts</a>
 
       <a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a>
 
       <a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a>
 +
                                                     
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="dropdown">
 
   <div class="dropdown">
 
     <button class="dropbtn">Modeling
 
     <button class="dropbtn">Modeling
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     <div class="dropdown-content-big">
 
     <div class="dropdown-content-big">
 
       <a href="/Team:IIT_Delhi/Model">Overview</a>
 
       <a href="/Team:IIT_Delhi/Model">Overview</a>
       <a href="/Team:IIT_Delhi/Database">Simulation Database</a>
+
       <a href="/Team:IIT_Delhi/Write_Model">Writing a Model</a>
 +
                                                        <a href="/Team:IIT_Delhi/Deterministic_Model">Deterministic Model </a>
 +
                                                        <a href="/Team:IIT_Delhi/Stochastic_Model">Stochastic Model</a>
 +
                                                        <a href="/Team:IIT_Delhi/Bifurcation">Bifurcation and Squareness</a>
 +
                                                        <a href="/Team:IIT_Delhi/Resources">Resource sharing</a>
 
     </div>
 
     </div>
 
     </div>
 
     </div>
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       <a href="/Team:IIT_Delhi/Engagement">Public Engagement</a>
 
       <a href="/Team:IIT_Delhi/Engagement">Public Engagement</a>
 
       <a href="/Team:IIT_Delhi/Collaborations">Collaborations</a>
 
       <a href="/Team:IIT_Delhi/Collaborations">Collaborations</a>
 +
                                                        <a href="/Team:IIT_Delhi/Safety">Safety</a>
 
     </div>
 
     </div>
 
   </div>  
 
   </div>  
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 +
                                                        <div class="dropdown">
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    <button class="dropbtn">Collaborations
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    <i class="fa fa-caret-down"></i>
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    </button>
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    <div class="dropdown-content">
 +
                                                        <a href="/Team:IIT_Delhi/Collaborations">Overview</a>
 +
      <a href="/Team:IIT_Delhi/GMM_legislation">GMM Legislation</a>
 +
      <a href="/Team:IIT_Delhi/berlin">iGEM Berlin</a>
 +
      <a href="/Team:IIT_Delhi/mohali">Mentoring IISER Mohali</a>
 +
      <a href="/Team:IIT_Delhi/glasgow">iGEM Glasgow</a>
 +
             
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    </div>
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  </div> 
 
   <div class="dropdown">
 
   <div class="dropdown">
 
     <button class="dropbtn">Notebook  
 
     <button class="dropbtn">Notebook  

Revision as of 12:23, 1 November 2017

iGEM IIT Delhi

RECIPES

                                                                                                                                                                                                                 

Accurate Experimenting for Accurate Results !!

     20x TAE buffer
  • 80mL of 1M Tris solution
  • 0.7264g of EDTANa2 salt
  • 2.284 mL glacial acetic acid
  • distilled H2O 100mL

  • For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
  • Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.
  • Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.

  • 50 mM glucose.
  • 25 mM Tris-Cl (pH 8.0).
  • 10 mM EDTA (pH 8.0).
    Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by autoclaving and store at 4°C.

  • 0.2 N NaOH (freshly diluted from a 10 N stock).
  • (100ul SDS + 700ul mq + 200ul NaOH) 1% (w/v) SDS.

  • 5 M potassium acetate, 60.0 ml
  • Glacial acetic acid, 11.5 ml
  • H2O, 28.5 ml
    The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. Store the solution at 4°C and transfer it to an ice bucket just before use.


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Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi