Difference between revisions of "Team:ZJU-China/Hardware/MediumWave"

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         <h1 id="bbp" class="ArticleHead GreenAH">Medium Wave</h1>
 
         <h1 id="bbp" class="ArticleHead GreenAH">Medium Wave</h1>
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        <p class="PP">For this year’s iGEM competition, our team has chosen to present Calcineurin-dependent response element (CDRE) (BBa_K2207021)and Mature serine protein from <em>Paecilomyces lilacinus</em> (BBa_K2207023) for the award of the basic part.</p>
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         <h2 class="H2Head" id="overview">Overview</h2>
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         <h2 class="H2Head" id="cdre">CDRE</h2>
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        <p class="PP">CDRE，calcineurin-dependent response element, is a segment of DNA sequence which can be regulated by specific transcription factor. This TF is activated by calcineurin in the present of calcium ion. To create a promoter can be up-regulated by calcium influx，we replaced the upstream activating sequence(UAS) of CYC1 promoter with 4 CDREs. This promoter is designed for Saccharomyces cerevisiae and it depends on S.cerevisiae's endogenous calcineurin and calmodulin<sup>[1][2]</sup>. We choose mRFP as the report gene. We cultured the yeast in calcium-inducing medium and uninduced medium which contains a relatively low concentration of calcium ion (1xYPD medium).</p>
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        <div class="imgdiv col-md-6 col-sm-6"><img class="textimg" style="height: 300px !important; width:auto !important;" src="https://static.igem.org/mediawiki/2017/1/19/ZJU_China_MWF_Rplot05.jpeg"></div>
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        <div class="imgdiv col-md-6 col-sm-6"><img class="textimg" style="height: 300px !important; width:auto !important;"  src="https://static.igem.org/mediawiki/2017/e/ea/ZJU_China_MWF_Rplot03.jpeg"></div>
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        <p class="capture">Fig.1 (a) Relative fluorescent intensity of two groups. (b) Relative calcium content in two groups</p>
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         <h2 class="H2Head" id="msp">Mature serine protein</h2>
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         <p class="PP">We want to transmit the signal to our <em>Trichoderma atroviride</em> directly by electromagnetic wave. So we need to produce a high-powered signal generator, to transmit our signals to <em>T.atroviride</em> remotely, which works as a “speaker”. In addition, it can be loaded on a UAV for large area use. For more information about our medium wave transduction, please <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Project/mt">click</a> to see more.</p>
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        <div class="imgdiv"><img class="textimg" src="https://static.igem.org/mediawiki/2017/0/08/ZJU_China_Hardware_zb0.jpg"></div>
  
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         <p class="PP">Root-knot nematodes (Meloidogyne spp.), which are one of the most destructive nematodes, cause the loss of crop about 10%, serious as high as 75%. Like the insect cuticlehe, nematode eggshell consists mainly of proteins and chitins. The egg-parasitic fungus <em>P.lilacinum</em> secretes protease and chitinase to hydrolyze the nematode eggshell, so that the root knot nematodes cannot grow normally<sup>[3]</sup>. Among them, serine protases plays an important role in hydrolyzing the eggshell of root-knot nematodes.</p>
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         <h2 class="H2Head" id="efforts">Our efforts</h2>
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         <p class="PP">Theoretically the frequency of the signal generator should be around 465kHZ<sup>[1]</sup>, and the power should be large enough. We first purchased an wave generator. But we didn’t have team members major in electrical engineering, we could not make suitable amplifiers. So its power was not enough, our experiment failed.</p>
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        <div class="imgdiv"><img class="textimg" src="https://static.igem.org/mediawiki/2017/f/fe/ZJU_China_temp_1.png"></div>
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        <p class="capture">Fig.2 Western-blot result</p>
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         <p class="PP" style="text-align: center !important;"><strong>Table of the Basic Parts we submitted to the BioBrick Registry</strong></p>
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        <table class="table">
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            <tr><th class="yellowTable">Name</th><th class="yellowTable">Type</th><th class="yellowTable">Description</th><th class="yellowTable">Designer</th><th class="yellowTable">Length(bp)</th><th class="yellowTable">Submitted</th></tr>
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            <tr><th class="grayTable">BBa_K2207003</th><td>Composite Parts</td><td>Trichoderma HR System I SOD-Tubulin dobule promoter</td><td>Yihe Zhang</td><td>3155</td><td>✔</td></tr>
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        </table>
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        <p class="capture">Table.OD600 reference point</p>
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        <div class="imgdiv"><img class="textimg" src="https://static.igem.org/mediawiki/2017/1/12/ZJU_China_Hardware_zb1.jpg"></div>
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        <p class="capture">Fig.1 The signal generator we purchased.</p>
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        <p class="PP">Then, we tried to make a Tesla coil, which is a kind of series resonant transformer, and can produce alternating magnetic field. We purchased the relative components from the Internet and assembled them.</p>
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        <p class="PP">We found that its power was sufficient, and even could light up a light bulb remotely.</p>
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        <div class="imgdiv"><img class="textimg" src="https://static.igem.org/mediawiki/2017/2/22/ZJU_China_Hardware_zb2.jpg"></div>
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        <p class="capture">Fig.2 The Tesla coil we made. It can even light up a light bulb.</p>
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        <p class="PP">But sadly, the heat effect of device was too significant that it could directly heat the whole medium even without Ferritin. Given more time, we would optimize this device and test our TRPV1-Ferritin system.</p>
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        <h2 class="H2Head" id="future">Future Work</h2>
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        <p class="PP">In the future, we will work with some professional experts and make better devices with smaller heating effect to test our medium wave transduction method. If the electromagnetic wave fails, we may try to use ultrasonic wave to combined with TRPC vibration-sensitive calcium channel to achieve remote control.</p>
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        <h2 class="H2Head" id="ref">Reference</h2>
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        <p class="PP ref">[1] Stanley S A, Gagner J E, Damanpour S, et al. Radio-Wave Heating of Iron Oxide Nanoparticles Can Regulate Plasma Glucose in Mice[J]. Science, 2012, 336(6081):604-8.</p>
  
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        <h2 class="H2Head" id="ref">Reference<hr></h2>
 
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        <p class="PP ref">[1] Cyert M S. Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stress[J]. Biochemical and biophysical research communications, 2003, 311(4): 1143-1150.</p>
 
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             <ul class="nav bs-docs-sidenav shorterli">
 
             <ul class="nav bs-docs-sidenav shorterli">
  
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                 <li><a href="#cdre">CDRE</a></li>
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                 <li><a href="#overview">Overview</a></li>
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                 <li><a href="#msp">Mature serine protein</a></li>
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                 <li><a href="#efforts">Our efforts</a></li>
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                <li><a href="#future">Future Work</a></li>
 
                 <li><a href="#ref">Reference</a></li>
 
                 <li><a href="#ref">Reference</a></li>
  

Revision as of 12:59, 1 November 2017

"

Medium Wave

Overview

We want to transmit the signal to our Trichoderma atroviride directly by electromagnetic wave. So we need to produce a high-powered signal generator, to transmit our signals to T.atroviride remotely, which works as a “speaker”. In addition, it can be loaded on a UAV for large area use. For more information about our medium wave transduction, please click to see more.

Our efforts

Theoretically the frequency of the signal generator should be around 465kHZ[1], and the power should be large enough. We first purchased an wave generator. But we didn’t have team members major in electrical engineering, we could not make suitable amplifiers. So its power was not enough, our experiment failed.

Fig.1 The signal generator we purchased.

Then, we tried to make a Tesla coil, which is a kind of series resonant transformer, and can produce alternating magnetic field. We purchased the relative components from the Internet and assembled them.

We found that its power was sufficient, and even could light up a light bulb remotely.

Fig.2 The Tesla coil we made. It can even light up a light bulb.

But sadly, the heat effect of device was too significant that it could directly heat the whole medium even without Ferritin. Given more time, we would optimize this device and test our TRPV1-Ferritin system.

Future Work

In the future, we will work with some professional experts and make better devices with smaller heating effect to test our medium wave transduction method. If the electromagnetic wave fails, we may try to use ultrasonic wave to combined with TRPC vibration-sensitive calcium channel to achieve remote control.

Reference

[1] Stanley S A, Gagner J E, Damanpour S, et al. Radio-Wave Heating of Iron Oxide Nanoparticles Can Regulate Plasma Glucose in Mice[J]. Science, 2012, 336(6081):604-8.





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