Line 63: | Line 63: | ||
<div class="page-title"><h5>Circuit Design</h5> | <div class="page-title"><h5>Circuit Design</h5> | ||
</div> <br /><br /><br /><br /><br /><br /> | </div> <br /><br /><br /><br /><br /><br /> | ||
− | <section><h3>The quest began with the search for ways to curb the problem | + | <section><h3>The quest began with the search for ways to curb the problem of capturing and detecting noxious gases and harmful chemicals. The task was to make a circuit with feedback positive and negative loops for increasing the sensitivity and specificity of detection. We came up with a novel circuit that can be modified easily for detecting various pollutants. Some pollutants activate, whereas others repress, a promoter. For eg. CO activates the COOA promoter whereas Acetaldehyde represses the XylR promoter. The circuit we have designed can be used for both kinds of pollutants.</h3> |
</section> | </section> | ||
<section><br /><br /><br /><br /> | <section><br /><br /><br /><br /> | ||
− | <h3>Circuit 1 | + | <h3>Circuit 1 and Circuit 2 represent circuits for activator and repressor type pollutants respectively.</h3> |
<center><div class="data-img"><img width="80%" src="https://static.igem.org/mediawiki/2017/9/99/T--IISER-Mohali-INDIA--circuit2.png" alt="Salicylate"></div> | <center><div class="data-img"><img width="80%" src="https://static.igem.org/mediawiki/2017/9/99/T--IISER-Mohali-INDIA--circuit2.png" alt="Salicylate"></div> | ||
<h4> Ciruit 1 <h4/> | <h4> Ciruit 1 <h4/> | ||
− | <h4> Different pollutants | + | <h4> Different pollutants with their activating circuits and respective promoters can be used to replace module 3 accordingly. Examples of pollutants acting as positive regulators include CO, Xylene, NO etc.</h4><br/><br/> |
<div src="data-img"> | <div src="data-img"> | ||
<img src="https://static.igem.org/mediawiki/2017/c/cc/T--IISER-Mohali-INDIA--circuit1.png" alt="Acetaldehyde" width="80%"> | <img src="https://static.igem.org/mediawiki/2017/c/cc/T--IISER-Mohali-INDIA--circuit1.png" alt="Acetaldehyde" width="80%"> | ||
<h4> Ciruit 2 <h4/> | <h4> Ciruit 2 <h4/> | ||
− | <h4> Different pollutants that can negatively regulate respective promoters can replace first part of module 3 | + | <h4> Different pollutants that can negatively regulate their respective promoters can replace the first part of module 3 accordingly.<h4/><br/> |
</center> | </center> | ||
</section> | </section> | ||
<br /> | <br /> | ||
<section> | <section> | ||
− | <h3> | + | <h3>The circuit can be divided into 3 parts. The bottom circuit gives a color when there is no pollutant. It |
− | signifies the number of cells alive and | + | signifies the number of cells alive and and capable of forming chromoprotein. In the presence of a |
− | pollutant, the topmost part detects the pollutant and activates the formation of second | + | pollutant, the topmost part detects the pollutant and activates the formation of a second |
− | chromoprotein in the middle circuit. Simultaneously, it inhibits the production of first | + | chromoprotein in the middle circuit. Simultaneously, it inhibits the production of the first |
chromoprotein. This results in the differential color formation depending upon the concentration of | chromoprotein. This results in the differential color formation depending upon the concentration of | ||
− | pollutant [1]. It has been documented that positive | + | pollutant [1]. It has been documented that positive feedbacks have stabilizing effects on circuits. Also, |
− | negative feedback loops | + | negative feedback loops make systems robust against alterations and noise [1].</h3> |
</section> | </section> | ||
Revision as of 13:21, 1 November 2017