Difference between revisions of "Team:KUAS Korea/Design"
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<p><font size=4>Also, the mechanism of heme detection using hrtR protein is as follows.</font></p> | <p><font size=4>Also, the mechanism of heme detection using hrtR protein is as follows.</font></p> | ||
| − | <p><font size=4> | + | <p><font size=4> ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> |
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.<br> | ② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.<br> | ||
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.<br> | ③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.<br> | ||
Revision as of 15:15, 1 November 2017
Design
POO-robiotics
Detection System 1: HssR/S Mechanism
The hssR and hssS mechanism of our project is as follows.
① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.
④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.
Detection System 2: HrtR Mechanism
Also, the mechanism of heme detection using hrtR protein is as follows.
① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.
