Difference between revisions of "Team:BNDS China"

Revision as of 15:33, 1 November 2017

Abstract

γ-Aminobutyric Acid (GABA) can be found in many plants and fermented foods and plays the role of inhibitory neurotransmitter in the human brain. However, high GABA concentrated products can only be produced through the complicated procedures. Our project aims to develop GABA-enriched probiotics including Escherichia coli Nissle 1917 through synthetic biology methods that may simplify the process of GABA production. In the bacterial way of GABA synthesis, two proteins, the glutamate: GABA antiporter (gadC) and the glutamate decarboxylase (gadA), form the glutamate decarboxylase (GAD) system. Though many bacteria incorporate these two genes, we select these genes from Lactobacillus brevis NCL912, a Lactic Acid Bacteria that is able to produce high amount of GABA with unique GAD sequences.

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-             <p>γ-Aminobutyric Acid (GABA) can be found in many plants and fermented foods, and plays the role of inhibitory neurotransmitter in human brain. Recently, GABA has been recognized as a natural relaxant as well as supplement to reduce anxiety levels and help combat stress. In order to reduce the cost of GABA production, our project aims to develop GABA-enriched yogurt through synthetic biology methods. Lactobacillus delbrueckii subsp. Bulgaricus is a common microorganism used in yogurt production and is able to produce GABA with low concentration. We propose to insert effective Lactobacillis promoters between gadR, gadA, and gadC (3 components of a high GABA producing species, Lactobacillus brevis NCL912). HPLC-UV measurement method will be used to evaluate the enhancement of GABA productivity in yogurt.</p>
+             <p>γ-Aminobutyric Acid (GABA) can be found in many plants and fermented foods and plays the role of inhibitory neurotransmitter in the human brain. However, high GABA concentrated products can only be produced through the complicated procedures. Our project aims to develop GABA-enriched probiotics including <em>Escherichia coli</em> Nissle 1917 through synthetic biology methods that may simplify the process of GABA production. In the bacterial way of GABA synthesis, two proteins, the glutamate: GABA antiporter (<em>gadC</em>) and the glutamate decarboxylase (<em>gadA</em>), form the glutamate decarboxylase (GAD) system. Though many bacteria incorporate these two genes, we select these genes from <em>Lactobacillus brevis</em> NCL912, a Lactic Acid Bacteria that is able to produce high amount of GABA with unique GAD sequences.</p>
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