Difference between revisions of "Team:Manchester/Description1"
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<h3><b>Introduction<b></h3><br> | <h3><b>Introduction<b></h3><br> | ||
| − | <p>This summer, we developed a system which could sequester and store high levels of phosphate: Phosphostore. In our project, | + | <p>Phosphate supplementation is essential for maximizing plant yields in many agricultural settings. However, phosphate runoff from fields often causes eutrophication, leading to the deterioration of aquatic ecosystems. Moreover phosphate rock is a finite and increasingly scarce resource. Together, these two issues suggest a closed-loop solution. This summer, we developed a system which could sequester and store high levels of phosphate: Phosphostore. In our project, we expressed Eut (Ethanolamine utilisation) bacterial microcompartments and a polyphosphate kinase (PPK) enzyme with a Pdu (1,2-propanediol utilisation) localization tag. This enables the encapsulation of the PPK enzyme within the microcompartment, |
| − | + | allowing the formation of polyphosphate chains to be built and stored safely from degradation by endogenous exopolyphosphatase (PPX). We were able to proof the concept of our project through fluorescent microscopy, which can be seen <a href="https://2017.igem.org/Team:Manchester/Demonstrate" target="_blank">here</a>.</p> | |
| − | allowing the formation of polyphosphate chains to be built and stored safely from degradation by endogenous exopolyphosphatase (PPX). | + | |
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| − | + | <h3><b>Real World<b></h3><br> | |
| + | <p> | ||
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Revision as of 17:37, 1 November 2017
Project Description
Introduction
Phosphate supplementation is essential for maximizing plant yields in many agricultural settings. However, phosphate runoff from fields often causes eutrophication, leading to the deterioration of aquatic ecosystems. Moreover phosphate rock is a finite and increasingly scarce resource. Together, these two issues suggest a closed-loop solution. This summer, we developed a system which could sequester and store high levels of phosphate: Phosphostore. In our project, we expressed Eut (Ethanolamine utilisation) bacterial microcompartments and a polyphosphate kinase (PPK) enzyme with a Pdu (1,2-propanediol utilisation) localization tag. This enables the encapsulation of the PPK enzyme within the microcompartment, allowing the formation of polyphosphate chains to be built and stored safely from degradation by endogenous exopolyphosphatase (PPX). We were able to proof the concept of our project through fluorescent microscopy, which can be seen here.
Real World
