Difference between revisions of "Team:Amazonas Brazil/Software"
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aspects related to this technique could be further improved to bring up this science | aspects related to this technique could be further improved to bring up this science | ||
revolution even more.</p> | revolution even more.</p> | ||
| − | <p class="p">In prokaryotes, the genome editing based on CRISPR/Cas9 machinery | + | <p class="p">In prokaryotes, the genome editing based on CRISPR/Cas9 machinery requires quite |
| − | laborious days of work in lab due to the multi-plasmid based edition. The system | + | laborious days of work in the lab due to the multi-plasmid based edition. The system is not standardized and, in some methodologies, more than one plasmid is demanded, |
| − | + | each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem consists | |
| − | each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem | + | |
in the addition of a linear DNA carrying homologous arms to provide a donor | in the addition of a linear DNA carrying homologous arms to provide a donor | ||
DNA to insert information into the genome during the HDR repair via. However, | DNA to insert information into the genome during the HDR repair via. However, | ||
| − | the linear donor DNA system has low edition efficiency due the action of DNA restriction | + | the linear donor DNA system has low edition efficiency due to the action of DNA restriction |
| − | systems present in wild type and E. coli strains, which can degrade the linear DNAs | + | systems present in wild-type and E. coli strains, which can degrade the linear DNAs |
before integration into genome. All this demands plenty of time and a lot of wet lab | before integration into genome. All this demands plenty of time and a lot of wet lab | ||
hours of work. To verify this information, we got in touch with other iGEM's teams who | hours of work. To verify this information, we got in touch with other iGEM's teams who | ||
Revision as of 17:55, 1 November 2017
CRISPeasy
Our perspective is to provide a bacterial genome editing vector based on BioBrick parts assembly easy to engineer as A, B, C… CRISPR.
What's the boost?
Scientific community have been achieved great results using CRISPR-Cas9 mediated genome editing techniques. While these methods are moving forward, some methodological aspects related to this technique could be further improved to bring up this science revolution even more.
In prokaryotes, the genome editing based on CRISPR/Cas9 machinery requires quite laborious days of work in the lab due to the multi-plasmid based edition. The system is not standardized and, in some methodologies, more than one plasmid is demanded, each one carrying a part of the CRISPR/Cas9 apparatus. Other critical problem consists in the addition of a linear DNA carrying homologous arms to provide a donor DNA to insert information into the genome during the HDR repair via. However, the linear donor DNA system has low edition efficiency due to the action of DNA restriction systems present in wild-type and E. coli strains, which can degrade the linear DNAs before integration into genome. All this demands plenty of time and a lot of wet lab hours of work. To verify this information, we got in touch with other iGEM's teams who had work with CRISPR/Cas9 in the previous years, you can check this outreach part with more details here.
