Attributions

In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.

• • Diethard Mattanovich, Brigitte Gasser, Michael Sauerand Hans Marx: organization of the iGEM project
• • Gabi Wilt ordered materials for our team
• • Corinna Rebnegger helped us doing flow cytometric experiments for characterization of our pGAL syn
• • Tom Gaßler advice on setting up our CRISPR/Cas9 mediated gene integration in S. cerevisiae by supporting us with primers for assembling the specific sgRNAs (adapted from Stovicek 2015).
• • Martin Altvater: provided us with different plasmids for Saccharomyces cerevisiae ready for our Golden Gate system
• • Jürgen Zanghellini supported us with modeling
• • Florian Strobl advice and lab material
• • Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids
• • Franziska Doleschal organized our bank account and managed all our invoices
• • Anna Gindl designed our Logo
• • Nikolaus Huber helped us setting up the core code of our wiki page
• • Sauer group provided us BB1_01 – BB1_08
• • Mattanovich/ Gasser group provided us material, all empty BBs for the Golden Gate system and many protocols:
  • • Preparation chemical competent E. coli
  • • Transformation chemical competent E. coli
  • • Preparation of electro competent S. cerevisiae
  • • Transformation of electro competent S. cerevisiae
  • • mRNA extraction
• • Bernd Albrecht helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters.
• • STV LBT helped us organizing the "Spritzerstand"

Furthermore, we want to thank Matthias Steiger, Alexandra Graf, Stefanie Wiesauer, Christiane Veit, Siniša Petrik, Roland Prielhofer, Hannes Rußmayer, Michael Egermeier, Valeria Ellena, Lina Heistinger, Dominik Jeschek, Richard Zahrl, Ursula Kieswetter and many more!