Cas9 & Cpf1 secretion
and activity
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| − | + | Professor of Regenerative Medicine<br> | |
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<div style="margin-top: 10px; font-weight: bold;">Bio</div> | <div style="margin-top: 10px; font-weight: bold;">Bio</div> | ||
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| − | + | Hello, I’m Niels Geijsen, and supervising the very first iGEM team of Utrecht University has been an immensely fun and inspiring adventure. Scientific challenges require for our brightest minds with diverse perspectives and backgrounds to come together. This is the Utrecht iGEM team: they are a group of smart, creative and ambitious students who have contributed a wide range of knowledge backgrounds, life experiences and hobbies to the project. It was such a fantastic experience to see how each member brought a special talent ranging from artistic drawing skills and video animation to photography, coding and web design. Together, they have truly outdone themselves. | |
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| + | As a professor in regenerative medicine at the Hubrecht Institute and Utrecht University, my research team works work at the boundaries of stem cell biology and technology development with a particular focus on (genetic) muscle disease. I spent 10 years in Boston, as post-doctoral fellow at the Whitehead Institute and faculty at Massachusetts General Hospital, Harvard Medical School and the Harvard Stem Cell Institute, and I'm particularly looking forward to joining the team in Boston for the Giant Jamboree. | ||
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| + | Aside from science, I experiment in the kitchen, have a passion for food and wine, and enjoy outdoor activities such as sailing, hiking and skiing. My three active young boys are a daily joy and challenge. <br> | ||
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Hi all, I am Roos Masereeuw, and since June 2015 I am full professor of Experimental Pharmacology at Utrecht Institute for Pharmaceutical Sciences in The Netherlands. I did a master’s in Biopharmaceutical Sciences at Leiden University, and my PhD in Pharmacology and Toxicology at Radboud University in Nijmegen (January 1997). | Hi all, I am Roos Masereeuw, and since June 2015 I am full professor of Experimental Pharmacology at Utrecht Institute for Pharmaceutical Sciences in The Netherlands. I did a master’s in Biopharmaceutical Sciences at Leiden University, and my PhD in Pharmacology and Toxicology at Radboud University in Nijmegen (January 1997). | ||
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Part of my PhD research and a postdoc period I did at National Institute for Environmental Sciences (NIEHS/NIH) in Research Triangle Park, North Carolina of USA. Between 1998 and 2015, I was first assistent and then associate professor at Radboud University Medical Centre in Nijmegen, after which I moved to Utrecht University. | Part of my PhD research and a postdoc period I did at National Institute for Environmental Sciences (NIEHS/NIH) in Research Triangle Park, North Carolina of USA. Between 1998 and 2015, I was first assistent and then associate professor at Radboud University Medical Centre in Nijmegen, after which I moved to Utrecht University. | ||
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My research is focused on understanding the pathways that can be pharmacologically triggered to enhance repair and regeneration processes after organ injury. An example is the development of a bioartificial kidney device to partly replace kidney function. Next to science, I mostly enjoy outdoor sports when I can. This includes mountain hiking, mountain biking and running, but I also love watching Netflix series with my family, and visiting concerts and festivals. | My research is focused on understanding the pathways that can be pharmacologically triggered to enhance repair and regeneration processes after organ injury. An example is the development of a bioartificial kidney device to partly replace kidney function. Next to science, I mostly enjoy outdoor sports when I can. This includes mountain hiking, mountain biking and running, but I also love watching Netflix series with my family, and visiting concerts and festivals. | ||
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Hello, I’m Guido van den Ackerveken and it is my privilege to be one of the supervisors of the energetic and bright team of Utrecht University. I am trained as a molecular biologist in the field of plant- and microbiology, and have always worked of plant-pathogen interactions. I have a research team of about 10 people and in our research we reveal molecular mechanisms in the dialogue between plants and microorganisms, which we then try to translate to improve the resistance of food crops to infectious diseases. It is real fun to work with the iGEM bunch and to support the starting researchers in their ambitious programme. | Hello, I’m Guido van den Ackerveken and it is my privilege to be one of the supervisors of the energetic and bright team of Utrecht University. I am trained as a molecular biologist in the field of plant- and microbiology, and have always worked of plant-pathogen interactions. I have a research team of about 10 people and in our research we reveal molecular mechanisms in the dialogue between plants and microorganisms, which we then try to translate to improve the resistance of food crops to infectious diseases. It is real fun to work with the iGEM bunch and to support the starting researchers in their ambitious programme. | ||
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Outside of my busy work I try to find time for playing squash and running (I ran my first marathon this year), but also of course to enjoy being with my family. Never a dull moment :-) | Outside of my busy work I try to find time for playing squash and running (I ran my first marathon this year), but also of course to enjoy being with my family. Never a dull moment :-) | ||
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I am a microbiologist and work at Utrecht University. My research interest focusses on two topics. One is to unravel the mechanisms involved in secretion of proteins and the role of secreted proteins in bacteria. This knowledge is important to understand how bacteria interact with their environment and can be used to optimize large scale production of proteins and vaccine development. | I am a microbiologist and work at Utrecht University. My research interest focusses on two topics. One is to unravel the mechanisms involved in secretion of proteins and the role of secreted proteins in bacteria. This knowledge is important to understand how bacteria interact with their environment and can be used to optimize large scale production of proteins and vaccine development. | ||
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My second topic of interest is teaching, and especially how to stimulate students to become independent and critical researchers. Therefore, assisting the first Utrecht iGEM team has been a great experience. In my spare time, I like to eat, watch movies and spend time with my family, which includes two daughters and a lazy cat. | My second topic of interest is teaching, and especially how to stimulate students to become independent and critical researchers. Therefore, assisting the first Utrecht iGEM team has been a great experience. In my spare time, I like to eat, watch movies and spend time with my family, which includes two daughters and a lazy cat. | ||
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Hello, my name is Clara (23) and I’m a first year Cancer, Stem Cells and Developmental biology Master student in Utrecht. I finished a Bachelor’s degree in Genetics in Barcelona last year and came to the Netherlands with a fellowship for post-graduate internships abroad, which allowed me to join Niels Geijsen’s lab for six months. | Hello, my name is Clara (23) and I’m a first year Cancer, Stem Cells and Developmental biology Master student in Utrecht. I finished a Bachelor’s degree in Genetics in Barcelona last year and came to the Netherlands with a fellowship for post-graduate internships abroad, which allowed me to join Niels Geijsen’s lab for six months. | ||
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I’m really happy to have been asked to write a piece as part of the team, even if I haven’t been there for the whole process. I joined the team during the summer period to help with some lab work that was being carried out in my former lab. It has been a great experience to help supervising the Utrecht iGEM team and its amazing people, full of joy and stress I have to admit. Now the deadline is getting closer and we are all preparing for the big final! Thank you, guys, for this opportunity! | I’m really happy to have been asked to write a piece as part of the team, even if I haven’t been there for the whole process. I joined the team during the summer period to help with some lab work that was being carried out in my former lab. It has been a great experience to help supervising the Utrecht iGEM team and its amazing people, full of joy and stress I have to admit. Now the deadline is getting closer and we are all preparing for the big final! Thank you, guys, for this opportunity! | ||
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Thank you! | Thank you! | ||
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| + | <script id="page-attributions" type="text/template"> | ||
| + | <div class="page-heading">BioBricks - Basic Parts</div> | ||
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| + | This page contains an overview of all the basic BioBricks we created for our project. | ||
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| + | <br><br> | ||
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| + | <table> | ||
| + | <tr> | ||
| + | <td>Number</td> | ||
| + | <td>Name</td> | ||
| + | <td>Short description</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | Ba_K2351000: parts.igem.org/Part:BBa_K2351000 Ig lambda-2 chain V region signal sequence Allows for proteins to be secreted | ||
| + | Ba_K2351001 | ||
| + | parts.igem.org/Part:BBa_K2351001 Best basic part: dAsCpf1 DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited. | ||
| + | Ba_K2351002 | ||
| + | parts.igem.org/Part:BBa_K2351002 nCas9 A cas9 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component that contains illegal restriction sites. | ||
| + | Ba_K2351003 | ||
| + | parts.igem.org/Part:BBa_K2351003 nCpf1 A cpf1 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component that contains illegal restriction sites. | ||
| + | Ba_K2351004 | ||
| + | parts.igem.org/Part:BBa_K2351004 Glycine [3x] linked histidine[6x] tag Basic histidine tag with preceding glycine linker | ||
| + | Ba_K2351007 | ||
| + | parts.igem.org/Part:BBa_K2351007 nCas9 A cas9 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component. | ||
| + | Ba_K2351008 | ||
| + | parts.igem.org/Part:BBa_K2351008 nCpf1 A cpf1 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component. | ||
| + | Ba_K23510011 | ||
| + | parts.igem.org/Part:BBa_K2351011 dCpf1 DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited. | ||
| + | Ba_K23510012 | ||
| + | parts.igem.org/Part:BBa_K2351012 sCpf1 Phytobrick* // Cpf1 that will be secreted from humane cells. | ||
| + | Ba_K23510013 | ||
| + | parts.igem.org/Part:BBa_K2351013 dCpf1 Phytobrick*// DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited. | ||
| + | Ba_K23510014 | ||
| + | parts.igem.org/Part:BBa_K2351014 sCas9 Phytobrick* // Cas9 that will be secreted from humane cells | ||
| + | |||
| + | |||
</script> | </script> | ||
Revision as of 18:34, 1 November 2017
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Comparison of endonuclease activity for Cas9 and Cpf1 that has been produced in, and excreted by, HEK293 cells.
MESA two-component system replication
Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.
OUTCASST system production
Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.
Modeling and
mathematics
mathematics
Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.
InterLab study participation
Results and details of our measurements for the iGEM 2017 InterLab Study.
Stakeholders & opinions
Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.
Risks & safety-issues
Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.
Design & integration
OUTCASST toolkit and product design with factors such as bio-safety and user-friendliness taken into account.
Outreach
Videos we made for the dutch public, together with 'de Kennis van Nu'.
Meet our team
About us, our interests and roles in the team and our supervisors.
Sponsors
A listing of our sponsors, how they assisted us and our gratitude for their assistance.
Collaborations
Read about our exchanges with other iGEM teams and government agencies.
Achievements
A short description of all that we have achieved during our participation in the iGEM.
Attributions
A thank-you for everyone that assited us, both in and outside the lab.
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